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VitelloTag: a tool for high-throughput cargo delivery into oocytes
D Nathaniel Clarke1, Akshay Kane2, Margherita Perillo2
1Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142,USA.
Development (Cambridge, England)
|August 22, 2024
Summary
VitelloTag offers a cost-effective, high-throughput method for delivering molecular tools into oocytes, overcoming limitations of traditional microinjection. This novel system utilizes vitellogenin-derived fusion proteins for efficient gene editing in developmental and reproductive biology research.
Area of Science:
- Developmental Biology
- Reproductive Biology
- Molecular Biology
- Biotechnology
Background:
- Delivering molecular tools into oocytes is crucial for research in developmental and reproductive biology.
- Conventional microinjection is labor-intensive, requires specialized equipment, and has low efficiency, especially for delicate oocytes or species with limited spawning seasons.
Purpose of the Study:
- To develop a novel, cost-effective, and high-throughput system for delivering molecular tools into oocytes.
- To overcome the limitations associated with traditional microinjection techniques in oocyte research.
Main Methods:
- Development of VitelloTag, a system utilizing vitellogenin-derived fusion proteins.
- Utilizing receptor-mediated endocytosis for efficient cargo delivery into oocytes.
- Application of VitelloTag for delivering Cas9/sgRNA complexes for gene knockout.
Main Results:
- VitelloTag provides a cost-effective and high-throughput solution for oocyte delivery.
- The system enables efficient cargo delivery via receptor-mediated endocytosis.
- Successful gene knockout was demonstrated in two distantly related species using VitelloTag.
Conclusions:
- VitelloTag represents a significant advancement in oocyte delivery methods for developmental and reproductive biology.
- The system offers a practical and efficient alternative to microinjection, broadening research possibilities.
- VitelloTag facilitates high-throughput gene editing in oocytes across diverse species.
