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Updated: Jun 15, 2025

An Orthotopic Bladder Cancer Model for Gene Delivery Studies
07:48

An Orthotopic Bladder Cancer Model for Gene Delivery Studies

Published on: December 1, 2013

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Anoikis-Related Genes Impact Prognosis and Tumor Microenvironment in Bladder Cancer

Tao Zhang1,2, Shaojun Chen3,2, Shanna Qu3,2

  • 1Department of Urology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250021, China.

Molecular Biotechnology
|August 22, 2024
PubMed

Insights

This study introduces an anoikis score to predict bladder cancer patient outcomes and immune microenvironment changes. A high anoikis score correlates with increased tumor cell proliferation and altered immune cell infiltration.

Area of Science:

  • Oncology
  • Cancer Metastasis
  • Immunology

Background:

  • Anoikis, or anchorage-dependent cell death, is crucial for preventing tumor colonization and metastasis.
  • While anoikis mechanisms are studied in solid tumors, their role in bladder cancer and impact on the tumor immune microenvironment are unclear.

Purpose of the Study:

  • To investigate the role of anoikis in bladder cancer.
  • To develop a prognostic model for bladder cancer based on anoikis-related genes.
  • To analyze the effect of anoikis on the bladder tumor immune microenvironment.

Main Methods:

  • Developed an anoikis score using five genes (ETV7, NGF, SCD, LAMC1, CASP6) from TCGA data.
  • Categorized patients into high- and low-risk groups based on the anoikis score.
  • Assessed bladder cancer cell proliferation in vitro.
  • Constructed a prognostic nomogram integrating the anoikis score with clinicopathological features.
  • Analyzed immune cell infiltration in relation to the anoikis score.

Main Results:

  • The anoikis score effectively categorized patients into distinct risk groups.
  • SCD was identified as a gene that enhances bladder cancer cell proliferation in vitro.
  • The prognostic nomogram demonstrated precision in predicting patient outcomes.
  • High anoikis score groups showed increased infiltration of Treg cells and M2 macrophages.
  • Reduced levels of CD8+ T cells were observed in the high anoikis score group.

Conclusions:

  • The developed anoikis score is a valuable predictor of bladder cancer prognosis and immune cell infiltration.
  • Anoikis plays a significant role in bladder cancer progression and immune modulation.
  • The findings suggest potential therapeutic strategies targeting anoikis in bladder cancer treatment.
Keywords:
AnoikisBladder cancerDrug responseImmune microenvironment

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