Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution00:52

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

1.4K
At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
1.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Massive barcode-free chemical screenings enable the discovery of bioactive macrocycles with passive membrane permeability.

Nature communications·2026
Same author

From sequence to function: Bridging single-molecule kinetics and molecular diversity.

Science (New York, N.Y.)·2026
Same author

Diffusion of DNA on Atomically Flat 2D Material Surfaces.

ACS nano·2025
Same author

Single-molecule parallel analysis for rapid exploration of sequence space.

Nature protocols·2025
Same author

Investigating Single-Molecule Molecular Inversion Probes for Medium-Scale Targeted DNA Methylation Analysis.

Epigenomes·2025
Same author

Disentangling Timescales of Molecular Kinetics with spFRET using ALEX-FCS.

Journal of fluorescence·2025
Same journal

A native sulfur deposit in Gale crater, Mars.

Science (New York, N.Y.)·2026
Same journal

Coordinated demise of harmful algal blooms.

Science (New York, N.Y.)·2026
Same journal

Genetic effects put into context.

Science (New York, N.Y.)·2026
Same journal

Bacteria share proteins to survive antibiotics.

Science (New York, N.Y.)·2026
Same journal

Impacts shaped Earth's first continents.

Science (New York, N.Y.)·2026
Same journal

Erratum for the Report "Covalently bonded single-molecule junctions with stable and reversible photoswitched conductivity" by C. Jia <i>et al</i>.

Science (New York, N.Y.)·2026
See all related articles

Related Experiment Video

Updated: Apr 16, 2026

Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System
12:30

Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System

Published on: February 9, 2017

12.1K

Single-molecule structural and kinetic studies across sequence space.

Ivo Severins1,2, Carolien Bastiaanssen1, Sung Hyun Kim1,3

  • 1Department of BioNanoScience, Kavli Institute of Nanoscience, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, the Netherlands.

Science (New York, N.Y.)
|August 22, 2024
PubMed
Summary
This summary is machine-generated.

We developed a new method, Single-molecule Parallel Analysis for Rapid eXploration of Sequence space (SPARXS), to efficiently study DNA sequences. SPARXS analyzes millions of molecules, revealing sequence-function relationships in processes like DNA recombination.

More Related Videos

Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web
09:51

Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web

Published on: July 16, 2017

15.4K
Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
07:19

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

12.6K

Related Experiment Videos

Last Updated: Apr 16, 2026

Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System
12:30

Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System

Published on: February 9, 2017

12.1K
Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web
09:51

Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web

Published on: July 16, 2017

15.4K
Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
07:19

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

12.6K

Area of Science:

  • Molecular Biology
  • Biophysics
  • Genetics

Background:

  • Understanding the relationship between DNA sequence, structure, and function is fundamental.
  • Single-molecule techniques offer dynamic insights but are often limited by assay throughput.
  • Screening large sequence libraries for function remains a bottleneck in molecular studies.

Purpose of the Study:

  • To introduce a high-throughput method, SPARXS, for analyzing sequence-dependent molecular dynamics.
  • To apply SPARXS to investigate the sequence-specific kinetics of the Holliday junction in homologous recombination.
  • To demonstrate SPARXS's capability in uncovering sequence patterns and constructing thermodynamic models.

Main Methods:

  • Integration of single-molecule fluorescence with next-generation sequencing.
  • Development of Single-molecule Parallel Analysis for Rapid eXploration of Sequence space (SPARXS).
  • Application to study millions of Holliday junction molecules across thousands of sequences.

Main Results:

  • SPARXS enables high-throughput analysis of molecular dynamics and sequence-function relationships.
  • The study revealed sequence patterns and motifs governing Holliday junction kinetics.
  • Successfully constructed thermodynamic models based on extensive sequence data.

Conclusions:

  • SPARXS is a versatile tool for high-throughput, sequence-based molecular analysis.
  • The method facilitates the study of sequence-specific mechanisms at the molecular level.
  • SPARXS advances the understanding of DNA recombination and other sequence-dependent biological processes.