Related Experiment Video
Updated: Jun 15, 2025
Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
Hydrogen-producing conditions and mutation mechanisms of a highly efficient mutant strain Ethanoligenens harbinense
Guoxiang Zheng1, Dongxu Tao2, Nanqi Ren3
1College of Engineering, Northeast Agriculture University, Harbin 150030, PR China; School of Environment, Harbin Institute of Technology, Harbin 150001, PR China; Key Laboratory of Swine Facilities Engineering, Ministry of Agriculture and Rural Affairs, Harbin 150030, PR China.
Abstract:
In this study, the optimal hydrogen (H2) production conditions of the high-efficiency H2-producing mutant strain Ethanoligenens harbinense YR-3 (carbon-nitrogen ratio 5.5, phosphate buffer 80 mM, initial pH 6.0, biotin 1.4 mg/L) are obtained by intermittent experiments. The maximum specific H2 production rate of YR-3 (2.85 mol H2/mol glucose) was 1.4 times that of the wild strain ZGX4 (2.04 mol H2/mol glucose). The liquid-phase products are mainly ethanol and acetic acid, indicating that the metabolic pathway has not changed. Two-dimensional electrophoresis and mass spectrometry were used to compare and analyze the protein map differences between YR-3 and ZGX4. The results show that 1,6-fructose diphosphate aldolase and the flavoprotein in hydrogenase are highly expressed. This study will provide a theoretical basis for the genetic modification of high-efficiency H2-producing strains and the improvement of H2 production capacity.
