Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Provider attitudes, practices, and barriers in alcohol-associated liver disease care: Findings from the CASL ALD SIG national survey.

Canadian liver journal·2026
Same author

Dissecting genetic variance structure and evaluating genomic prediction models for single-cross hybrids derived from Stiff Stalk and Non-Stiff Stalk maize heterotic groups.

G3 (Bethesda, Md.)·2026
Same author

The journey after restart: understanding the multidimensional experience of cardiac arrest survivors.

Singapore medical journal·2026
Same author

Prognostic performance of plasma and urinary biomarkers of kidney injury and function after out-of-hospital cardiac arrest.

Resuscitation·2026
Same author

Fouling-Free Inertial Microfluidic Cell Clarification for Lentivirus Manufacturing.

Biotechnology and bioengineering·2026
Same author

Cardiometabolic multimorbidity and survival after out-of-hospital cardiac arrest.

Resuscitation plus·2026

Related Experiment Video

Updated: Jun 15, 2025

Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses
09:43

Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses

Published on: March 8, 2024

1.6K

iSECRETE: Integrating Microfluidics and DNA Proximity Amplification for Synchronous Single-Cell Activation and IFN-γ

Ri Lu1,2, Yan Shan Ang3, Ka-Wai Cheung1

  • 1Critical Analytics for Manufacturing of Personalised Medicine IRG, Singapore-MIT Alliance for Research and Technology, Singapore, 138602, Singapore.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|August 23, 2024
PubMed
Summary

Introducing iSECRETE, a rapid, wash-free platform for single-cell cytokine secretion analysis. This integrated system provides fast, accurate detection of immune responses, advancing biological research.

Keywords:
CAR T cellscytokinesdeterministic lateral displacementimmune profilingmicrofluidics

More Related Videos

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

14.9K
A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
12:21

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

10.6K

Related Experiment Videos

Last Updated: Jun 15, 2025

Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses
09:43

Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses

Published on: March 8, 2024

1.6K
A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

14.9K
A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
12:21

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

10.6K

Area of Science:

  • Immunology
  • Biotechnology
  • Cellular Biology

Background:

  • Cytokine dysregulation significantly impacts immune modulation and disease progression.
  • Current cytokine profiling methods are often slow, labor-intensive, and fail to capture dynamic immune responses.

Purpose of the Study:

  • To develop an integrated platform, iSECRETE, for rapid, single-cell cytokine secretion analysis.
  • To enable synchronous cell activation and target-responsive protein detection within a short timeframe.

Main Methods:

  • Utilized a multifunctional microfluidic system incorporating a DNA proximity assay (DPA).
  • Achieved one-pot homogenous cytokine signal amplification for sensitive detection.
  • Demonstrated wash-free, target-responsive protein detection at room temperature.

Main Results:

  • iSECRETE provides single-cell IFN-γ cytokine secretion analysis within 30 minutes.
  • Achieved a limit of detection of approximately 50 secreted molecules per cell.
  • Successfully detected single-cell IFN-γ secretion from CAR T cells upon cancer target exposure within 6 hours.

Conclusions:

  • iSECRETE is the fastest single-cell sample-to-result cytokine secretion assay to date.
  • The platform offers a powerful tool for understanding biological phenotypes, functions, and pathways under in vivo-like conditions.
  • Enables robust analysis across various sample types.