Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Simultaneous three-photon and optical coherence microscopy deep within an intact mouse brain.

Npj imaging·2026
Same author

Three photon microscopy of mouse brain structure and function at 2 mm depth and beyond.

bioRxiv : the preprint server for biology·2026
Same author

Erratum: Efficient, broadly-tunable, hollow-fiber source of megawatt pulses for multiphoton microscopy: erratum.

Biomedical optics express·2026
Same author

Scattering-enabled epi-quantitative phase imaging reveals subcellular detail in organoids and deep mouse brains.

bioRxiv : the preprint server for biology·2026
Same author

A temporal and spatial atlas of adaptive immune responses in the lymph node following viral infection.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

A subnanolitre tetherless optoelectronic microsystem for chronic neural recording in awake mice.

Nature electronics·2025

Related Experiment Video

Updated: Jun 15, 2025

Multiphoton Intravital Imaging for Monitoring Leukocyte Recruitment during Arteriogenesis in a Murine Hindlimb Model
07:50

Multiphoton Intravital Imaging for Monitoring Leukocyte Recruitment during Arteriogenesis in a Murine Hindlimb Model

Published on: September 30, 2021

1.4K

Multiphoton fluorescence microscopy for in vivo imaging.

Chris Xu1, Maiken Nedergaard2, Deborah J Fowell3

  • 1School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14850, USA.

Cell
|August 23, 2024
PubMed
Summary

Multiphoton fluorescence microscopy (MPFM) enables high-resolution deep-tissue imaging by overcoming light scattering. This versatile technique is crucial for studying biological processes in neuroscience, cancer biology, and immunology.

More Related Videos

In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin
05:45

In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin

Published on: July 11, 2019

7.4K
Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy
10:41

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy

Published on: June 7, 2019

8.4K

Related Experiment Videos

Last Updated: Jun 15, 2025

Multiphoton Intravital Imaging for Monitoring Leukocyte Recruitment during Arteriogenesis in a Murine Hindlimb Model
07:50

Multiphoton Intravital Imaging for Monitoring Leukocyte Recruitment during Arteriogenesis in a Murine Hindlimb Model

Published on: September 30, 2021

1.4K
In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin
05:45

In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin

Published on: July 11, 2019

7.4K
Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy
10:41

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy

Published on: June 7, 2019

8.4K

Area of Science:

  • Biomedical Optics
  • Microscopy
  • Biological Imaging

Background:

  • Biological tissues scatter light, limiting deep-tissue imaging with conventional microscopy.
  • Nonlinear excitation in multiphoton fluorescence microscopy (MPFM) confines fluorescence emission to the focal volume, reducing scattering.
  • MPFM allows high-resolution imaging deep within intact biological tissues.

Purpose of the Study:

  • To present the principles, implementations, capabilities, and limitations of MPFM.
  • To review key applications of MPFM in neuroscience, cancer biology, and immunology.
  • To highlight MPFM's role in advancing biological research.

Main Methods:

  • Nonlinear optical excitation for fluorescence generation.
  • Confocal detection to isolate focal volume emission.
  • Review of existing literature and case studies.

Main Results:

  • MPFM overcomes scattering for deep-tissue imaging.
  • High-resolution imaging of cellular and subcellular structures in native environments is achieved.
  • Significant contributions to neuroscience, cancer biology, and immunology research.

Conclusions:

  • MPFM is an indispensable tool for in vivo biological imaging.
  • Its versatility drives discoveries in complex biological processes.
  • MPFM continues to expand the possibilities for structural and functional studies.