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Updated: Jun 15, 2025

Study of Protein-protein Interactions in Autophagy Research
14:08

Study of Protein-protein Interactions in Autophagy Research

Published on: September 9, 2017

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Function of CSNK2/CK2 selectively affects the endoplasmic reticulum and the Golgi apparatus in mtor-mediated

Pablo Sanz-Martinez1,2, Rayene Berkane1,2, Alexandra Stolz1,2

  • 1Institute of Biochemistry 2 (IBC2), Goethe University, Frankfurt am Main, Germany.

Autophagy
|August 23, 2024
PubMed

Insights

Selective autophagy, or ER-phagy, maintains endoplasmic reticulum (ER) homeostasis. CSNK2 inhibition impacts ER-phagy, while ATR inhibition affects multiple organelle autophagy pathways.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Autophagy Research

Background:

  • Selective autophagy, including reticulophagy/ER-phagy, is crucial for endoplasmic reticulum (ER) homeostasis.
  • Autophagy receptors like RETREG/FAM134 are regulated by phosphorylation-dependent ubiquitination.
  • Kinases downstream of MTOR, such as ATR and CSNK2/CK2, influence reticulophagy flux.

Purpose of the Study:

  • To investigate the global proteomics changes upon inhibition of CSNK2 and ATR.
  • To elucidate the specific roles of CSNK2 and ATR in selective autophagy pathways.

Main Methods:

  • Global proteomics analysis.
  • Inhibition of CSNK2 using SGC-CK2-1.
  • Inhibition of ATR using VE-822.
  • Analysis of regulatory ubiquitination and receptor clustering.

Main Results:

  • CSNK2 inhibition prevented regulatory ubiquitination of RETREG1 and RETREG3, and formation of RETREG clusters.
  • CSNK2 inhibition's effects were primarily limited to ER/reticulophagy and Golgi/Golgiphagy.
  • ATR inhibition (ATRi) broadly impacted most organelle/selective autophagy pathways.

Conclusions:

  • CSNK2 plays a specific role in ER/reticulophagy and Golgi/Golgiphagy regulation.
  • ATR has a more widespread role, affecting the majority of selective autophagy pathways.
  • These findings provide resource data for understanding kinase regulation of selective autophagy.
Keywords:
Kinase signalingLAMP1RETREGmethuosisselective autophagyvacuolization

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