Oxidative modulations in platelets stored in SSP+, PAS-G and Tyrode's buffer: a comparative analysis.

Area of Science:

• Hematology
• Biochemistry
• Biomedical Science

Background:

• Platelet additive solutions (PASs) enhance stored platelet efficacy.
• Oxidative stress induces storage lesions, impairing platelet function.
• Limited research exists on oxidative stress impact on platelets in PASs.

Purpose of the Study:

• To compare platelet variations in different storage solutions: SSP+, PAS-G, and Tyrode's buffer.
• To assess the influence of oxidative stress on stored platelets in various PASs.

Main Methods:

• Wistar rat platelets were stored for seven days at 22°C in SSP+, PAS-G, or Tyrode's buffer.
• Analyzed platelet metabolism, function, oxidative stress, antioxidant status, and viability on days 1, 3, 5, and 7.

Main Results:

• SSP+ maintained platelet function, viability, and antioxidant defenses (SOD, CAT, GSH), reducing lipid peroxidation.
• PAS-G improved platelet function and antioxidant defenses (SOD, GSH), protecting lipids and thiols from oxidation.
• Tyrode's buffer showed increased SOD and GSH, with preserved lipids and thiols.

Conclusions:

• SSP+ and PAS-G are more effective than Tyrode's buffer in maintaining platelet efficacy until Day 7.
• PAS-G and SSP+ demonstrate superior platelet response to oxidative stress during storage.
• This study provides a comprehensive comparison of PAS influence on platelet oxidative stress responses.