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Caffeic Acid Alleviates Oxidative Stress and Prolongs the Shelf-Life of Rat Platelets
Magdaline Christina Rajanand1, Anusha Berikai Ananthakrishna1, Vani Rajashekaraiah1
1Department of Biotechnology and Genetics, School of Sciences, JAIN (Deemed-to-be University), Bengaluru, India.
Biopreservation and Biobanking
|June 5, 2025
Summary
Caffeic acid (CA) preserves stored platelet quality by reducing oxidative stress and lipid peroxidation. This antioxidant additive maintained platelet viability and function, extending shelf-life up to 7 days.
Area of Science:
- Biochemistry
- Hematology
- Biotechnology
Background:
- Oxidative stress significantly contributes to platelet storage lesions.
- Antioxidant additives can mitigate oxidative damage in stored platelets.
- Caffeic acid (CA) is a potent antioxidant with ROS scavenging and anti-lipid peroxidation properties.
Purpose of the Study:
- To investigate the efficacy of caffeic acid (CA) as an additive in platelet storage solutions.
- To evaluate the impact of CA on oxidative stress, platelet function, and viability during storage.
Main Methods:
- Rat platelets were stored in SSP+ with varying CA concentrations for 7 days.
- Optimal CA concentration (5 µM) was selected based on ROS inhibition.
- Platelet function, viability, oxidative stress markers, and antioxidant defenses were analyzed over 11 days.
Main Results:
- 5 µM CA maximally inhibited ROS by day 7 and scavenged superoxide radicals.
- CA supplementation enhanced antioxidant defenses and reduced lipid peroxidation.
- Platelet activation, aggregation, and microbial contamination decreased with CA; viability and metabolism were maintained.
Conclusions:
- Caffeic acid (5 µM) effectively preserves stored platelet quality until day 7.
- CA shows potential as an additive to extend platelet shelf-life and maintain efficacy.
- This study highlights CA's role in mitigating platelet storage lesions.
