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Visual detection of biogenic monoamines based on amine oxidase-peroxidase coupling reaction using ascorbic acid as

Young-Chang Kim1, Jun-Mo Ha1, Jaeick Lee1

  • 1Department of Food and Biotechnology, Korea University, Sejong, 30019 Republic of Korea.

Food Science and Biotechnology
|August 26, 2024
PubMed

Keywords:
Amine oxidaseAscorbic acidBiogenic aminesDoenjangVisual detection

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Amines to Sulfonamides: The Hinsberg Test01:23

Amines to Sulfonamides: The Hinsberg Test

The Hinsberg test is a method to identify primary, secondary and tertiary amines, named after its pioneer, Oscar Hinsberg. Here, amines are treated with benzenesulfonyl chloride, also known as the Hinsberg reagent, in the presence of an excess of aqueous base, followed by acidification. Based on the nature of the amines, different changes are observed.
Generally, a primary amine reacts with the Hinsberg reagent to produce an N-substituted benzenesulfonamide. The electron-withdrawing sulfonyl...