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Updated: Jun 20, 2026

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

Published on: December 10, 2016

Emerging Clostridioides difficile ribotypes have divergent metabolic phenotypes.

Firas S Midani^1,2, Heather A Danhof^1,2, Nathanael Mathew^1,2

¹Alkek Center for Metagenomics and Microbiome Research, Baylor College of Medicine, Houston, Texas, USA.

Biorxiv : the Preprint Server for Biology

|August 26, 2024

Summary

Clostridioides difficile (C. difficile) is a versatile gut pathogen. This study reveals lineage-specific metabolic differences in C. difficile strains, impacting their adaptation and potential therapeutic strategies.

Keywords:

Clostridioides difficile carbon metabolism growth modeling ribotyping

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Area of Science:

Microbiology
Pathogen Metabolism
Genomics

Background:

Clostridioides difficile is a major cause of diarrheal infections.
Genetic diversity exists among C. difficile strains, with specific ribotypes dominating human infections.
Metabolic adaptations of epidemic C. difficile ribotypes remain largely unexplored.

Purpose of the Study:

To investigate carbon substrate utilization in a diverse collection of C. difficile clinical isolates.
To identify metabolic differences linked to specific ribotypes and phylogenetic lineages.
To understand the metabolic basis for the emergence and adaptation of C. difficile.

Main Methods:

Carbon substrate utilization profiling of 88 C. difficile clinical isolates.
Growth assays across 22 different ribotypes.
Phylogenetic analysis to correlate metabolic capabilities with genetic lineage.

Main Results:

C. difficile exhibits generalist metabolic capabilities, utilizing a wide range of carbon sources.
Metabolic phenotypes clustered by phylogenetic relationship, showing ribotype- and clade-specific differences.
Emerging lineages (ribotypes 023, 255) displayed divergent metabolic profiles.
The most evolutionarily distant clade (Clade 5) showed robust growth on simple sugars.

Conclusions:

C. difficile employs a generalist metabolic strategy, but with significant lineage-specific adaptations.
Metabolic diversity is crucial for understanding C. difficile evolution and pathogenesis.
Findings support the rational design of targeted therapeutic interventions by exploiting metabolic niches.