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Updated: Jun 15, 2025
Author Spotlight: Quantifying Rough Eye Phenotypes in Drosophila Models of Amyotrophic Lateral Sclerosis with Frontotemporal Dementia
Published on: October 4, 2024
Flynotyper 2.0: An updated tool for rapid quantitative assessment of Drosophila eye phenotypes
Johnathan Ray1, Deepro Banerjee1,2, Qingyu Wang1,2
1Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA.
Abstract:
About two-thirds of the genes in the Drosophila melanogaster genome are also involved in its eye development, making the Drosophila eye an ideal system for genetic studies. We previously developed Flynotyper, a software that uses image processing operations to identify and quantify the degree of roughness by measuring disorderliness of ommatidial arrangement in the fly eye. This software has enabled researchers to quantify morphological defects of thousands of eye images caused by genetic perturbations. Here, we present Flynotyper 2.0, a software that has an updated computer vision library, improved performance, and a streamlined pipeline for high-throughput analysis of multiple eye images. We also tested several batches of Drosophila eye images to ensure robustness and reproducibility of the updated Flynotyper 2.0 software.
Availability And Implementation:
The source code for Flynotyper 2.0 can be downloaded and installed from https://github.com/girirajanlab/flynotyper-desktop-application.
