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Related Experiment Video

Updated: Jun 15, 2025

Minimally-invasive Technique for Injection into Rat Optic Nerve

Minimally-invasive Technique for Injection into Rat Optic Nerve

Published on: May 19, 2015

Serotonin neuromodulation directs optic nerve regeneration.

Kristian Saied-Santiago¹, Melissa Baxter¹, Jaffna Mathiaparanam¹

¹Department of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, 19104, United States of America.

Biorxiv : the Preprint Server for Biology

|August 26, 2024

Summary

Serotonin (5-HT) signaling inhibits optic nerve regeneration in zebrafish by activating 5-HT1 receptors. Blocking these receptors promotes axonal regrowth, revealing a novel target for enhancing optic nerve repair.

Keywords:

5HT Serotonin axon regeneration optic nerve zebrafish

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Last Updated: Jun 15, 2025

Minimally-invasive Technique for Injection into Rat Optic Nerve

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Published on: May 19, 2015

Methods for Experimental Manipulations after Optic Nerve Transection in the Mammalian CNS

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Published on: May 12, 2011

Partial Optic Nerve Transection in Rats: A Model Established with a New Operative Approach to Assess Secondary Degeneration of Retinal Ganglion Cells

Partial Optic Nerve Transection in Rats: A Model Established with a New Operative Approach to Assess Secondary Degeneration of Retinal Ganglion Cells

Published on: October 15, 2017

Area of Science:

Neuroscience
Regenerative Medicine
Molecular Biology

Background:

Mammalian optic nerve regeneration is hindered by inhibitory factors, limiting therapeutic strategies.
Identifying pro-regenerative pathways is crucial for developing treatments for optic nerve injuries.

Purpose of the Study:

To identify pathways that promote optic nerve regeneration using larval zebrafish as a model.
To investigate the role of serotonin (5-HT) signaling in optic nerve regeneration.

Main Methods:

Conducted a small molecule screen to identify modulators of 5-HT signaling affecting optic nerve regeneration.
Utilized gene expression analysis to identify serotonin type-1 receptor genes in retinal ganglion cells (RGCs).
Performed experiments to inhibit 5-HT1 receptors and assess their impact on axonal regeneration.

Main Results:

Serotonin (5-HT) signaling pathways, specifically 5-HT1 receptors, were identified as inhibitors of optic nerve regeneration.
Inhibiting 5-HT1 receptors or 5-HT pathway components selectively impeded optic nerve regeneration.
5-HT1 receptor signaling is critical for initial axonal emergence from the injury site but not for later regeneration stages.
Activation of 5-HT1 receptors promoted enhanced and ectopic axonal regrowth.

Conclusions:

Serotonin-dependent neuromodulation plays a critical role in directing optic nerve regeneration in vivo.
Targeting 5-HT1 receptor signaling presents a potential therapeutic strategy for promoting optic nerve repair.