Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Anatomy of the Eyeball01:20

Anatomy of the Eyeball

6.7K
The eye is a spherical, hollow structure composed of three tissue layers. The outer layer — the fibrous tunic, comprises the sclera — a white structure — and the cornea, which is transparent. The sclera encompasses some of the ocular surface, most of which is not visible. However, the 'white of the eye' is distinctively visible in humans compared to other species. The cornea, a clear covering at the front of the eye, enables light penetration. The eye's middle...
6.7K
Photoreceptors and Visual Pathways01:22

Photoreceptors and Visual Pathways

5.9K
At the molecular level, visual signals trigger transformations in photopigment molecules, resulting in changes in the photoreceptor cell's membrane potential. The photon's energy level is denoted by its wavelength, with each specific wavelength of visible light associated with a distinct color. The spectral range of visible light, classified as electromagnetic radiation, spans from 380 to 720 nm. Electromagnetic radiation wavelengths exceeding 720 nm fall under the infrared category,...
5.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Automated dual-camera DMD system for stereo optical sectioning imaging.

Biomedical optics express·2026
Same author

Stereo slit-scanning tomography of the anterior segment of the human eye.

Scientific reports·2024
Same author

Pharmacological expansion of type 2 alveolar epithelial cells promotes regenerative lower airway repair.

Proceedings of the National Academy of Sciences of the United States of America·2024
Same author

Visualizing features with wide-field volumetric OCT angiography.

Optics express·2024
Same author

Deep learning-based target tracking with X-ray images for radiotherapy: a narrative review.

Quantitative imaging in medicine and surgery·2024
Same author

Real-time line-field optical coherence tomography for cellular resolution imaging of biological tissue.

Biomedical optics express·2024

Related Experiment Video

Updated: Jun 15, 2025

Author Spotlight: Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration
10:14

Author Spotlight: Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration

Published on: May 26, 2023

3.1K

Optical Assessment of Photoreceptor Function Over the Macula.

Shuibin Ni1,2, Shanjida Khan1,2, Alfonso Jiménez-Villar1

  • 1Casey Eye Institute, Oregon Health & Science University, Portland, OR, USA.

Translational Vision Science & Technology
|August 26, 2024
PubMed
Summary

This study introduces ultrahigh-speed swept-source optical coherence tomography (UHS-SS-OCT) with the SSADOR algorithm for advanced photoreceptor imaging. This new method offers sensitive, objective, and quantifiable macular visual function measurements for retinal disease monitoring.

More Related Videos

Measurement of Carotenoids in Perifovea using the Macular Pigment Reflectometer
09:35

Measurement of Carotenoids in Perifovea using the Macular Pigment Reflectometer

Published on: January 29, 2020

7.8K
Using Retinal Imaging to Study Dementia
09:17

Using Retinal Imaging to Study Dementia

Published on: November 6, 2017

21.6K

Related Experiment Videos

Last Updated: Jun 15, 2025

Author Spotlight: Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration
10:14

Author Spotlight: Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration

Published on: May 26, 2023

3.1K
Measurement of Carotenoids in Perifovea using the Macular Pigment Reflectometer
09:35

Measurement of Carotenoids in Perifovea using the Macular Pigment Reflectometer

Published on: January 29, 2020

7.8K
Using Retinal Imaging to Study Dementia
09:17

Using Retinal Imaging to Study Dementia

Published on: November 6, 2017

21.6K

Area of Science:

  • Ophthalmology
  • Biomedical Engineering
  • Medical Imaging

Background:

  • Assessing macular visual function is crucial for diagnosing and monitoring retinal diseases.
  • Current methods for evaluating photoreceptor function can be limited in speed, scope, or objectivity.
  • Next-generation imaging techniques are needed for early detection and precise management of visual impairments.

Purpose of the Study:

  • To develop and validate a novel functional photoreceptor imaging technique using ultrahigh-speed swept-source optical coherence tomography (UHS-SS-OCT) and the split-spectrum amplitude-decorrelation optoretinography (SSADOR) algorithm.
  • To enable rapid, non-contact, objective, and quantifiable measurements of macular visual function.
  • To advance the assessment of photoreceptor light response over a large retinal area.

Main Methods:

  • A UHS-SS-OCT prototype with a 1 MHz A-scan rate was developed.
  • A functional scanning protocol recorded 5 volumes in 3 seconds, using a selective flash pattern.
  • The SSADOR algorithm quantified photoreceptor light response by analyzing OCT signal changes in the outer segment before and after light stimulation.

Main Results:

  • The study enrolled 16 eyes from 8 subjects, successfully measuring photoreceptor light response.
  • A record field of view (3 × 3 mm²) with high topographical resolution (~100 µm) was achieved.
  • The SSADOR signal correlated with the flash pattern and strength, demonstrating consistency and reproducibility.

Conclusions:

  • The integration of UHS-SS-OCT and SSADOR enables comprehensive characterization of photoreceptor function over a clinically relevant field of view.
  • This approach allows for sensitive detection of changes in photoreceptor light response and focal impairments.
  • The workflow is suitable for integration into routine clinical practice and trials for retinal disease management.