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Updated: Jun 15, 2025
Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity
Published on: May 1, 2018
Phage display for discovery of anticancer antibodies
Polina V Istomina1, Andrey A Gorchakov2, Chatchanok Paoin3
1Molecular Biotechnology Laboratory, School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Suranaree, Muang, 111 University Avenue, Nakhon Ratchasima 30000, Thailand.
Abstract:
Antibodies and antibody-based immunotherapeutics are the mainstays of cancer immunotherapy. Expanding the repertoire of cancer-specific and cancer-associated epitopes targetable with antibodies represents an important area of research. Phage display is a powerful approach allowing the use of diverse antibody libraries to be screened for binding to a wide range of targets. In this review, we summarize the basics of phage display technology and highlight the advances in anticancer antibody identification and modification via phage display platform. Finally, we describe phage display-derived anticancer monoclonal antibodies that have been approved to date or are in clinical development.
Insights
Phage display technology enables the discovery and development of novel cancer immunotherapies. This review covers advances in identifying and modifying anticancer antibodies using phage display, including approved and investigational therapies.
Area of Science:
- Immunology
- Biotechnology
- Oncology
Background:
- Antibodies and antibody-based therapies are crucial in cancer immunotherapy.
- Identifying new cancer targets for antibody therapies is a key research area.
- Phage display is a versatile platform for screening antibody libraries against diverse targets.
Purpose of the Study:
- To review the fundamentals of phage display technology.
- To highlight advancements in anticancer antibody discovery and engineering using phage display.
- To discuss phage display-derived monoclonal antibodies in clinical use or development.
Main Methods:
- Summarizing the basics of phage display technology.
- Reviewing advances in antibody identification and modification via phage display.
- Compiling information on approved and investigational phage display-derived anticancer antibodies.
Main Results:
- Phage display is a powerful tool for identifying and engineering antibodies.
- Numerous anticancer antibodies have been developed using this platform.
- Several phage display-derived antibodies are approved or in clinical trials.
Conclusions:
- Phage display significantly contributes to the development of novel cancer immunotherapies.
- The platform facilitates the identification and optimization of targeted anticancer antibodies.
- Ongoing research promises further advancements in antibody-based cancer treatments.
