Related Experiment Video
Updated: Jun 15, 2025
Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System
Published on: August 25, 2022
[Effect of Collagen Peptides on Function of Mouse T Lymphocytes under Simulated Microgravity]
Shao-Yan Si1, Ya-Ya Qin1, Ying-Ying Wu1
1Department of Comprehensive Basic Experiment (Division Two), Strategic Support Force Medical Center, Beijing 100101, China.
Objective:
To understand the effect of collagen peptides on the function of mouse lymphocytes under simulated microgravity.
Methods:
The splenocytes of mice were isolated, and the rotary cell culture system was used to simulate the microgravity. The T lymphocytes were stimulated with mitotic agents, concanavalin A (ConA), and the cells were treated with different concentrations of collagen peptides. The proliferation of lymphocytes and the levels of cytokines in the supernatant were detected.
Results:
Simulated microgravity could inhibit the proliferation of spleen T lymphocytes and decrease the level of cytokines in the supernatant. Collagen peptides could promote the lymphocyte proliferation and cytokine production in cells cultured under simulated microgravity.
Conclusion:
Collagen peptides may attenuate the inhibitory effect of simulated microgravity on T lymphocytes by regulating the cell proliferation and the secretion of cytokines.
