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Updated: Jun 15, 2025
Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile
Published on: June 13, 2016
Circulating Exosomal microRNA Profiles Associated with Risk of Postoperative Recurrence in Chronic Rhinosinusitis
Siyuan Chen1,2, Jia Liu1,2, Zhili Feng1,2
1Department of Otolaryngology Head and Neck Surgery, The Affiliated Changsha Central Hospital, Hengyang Medical School, University of South China, Changsha, Hunan, People's Republic of China.
Background:
Exosomes carry various types of transcripts and serve as promising biomarkers for inflammatory diseases. However, the role of serum exosomal microRNAs (miRNAs) in chronic rhinosinusitis with nasal polyps (CRSwNP) is poorly clarified.
Methods:
A prospective exploratory cohort of 10 CRSwNP patients was conducted, and the serum exosome samples were subjected to miRNA sequencing. Two independent prospective cohorts, consisting of 40 and 54 patients respectively, were recruited from different medical centers for validation. These cohorts were monitored for over two years, with postoperative recurrence serving as the primary outcome measure. The top 3 differentially exosomal miRNAs were validated in the serum samples, and their predictive values for recurrence were assessed.
Results:
Eight CRSwNP patients completed the follow-up, comprising 4 non-recurrent cases and 4 recurrent cases. Distinctive profiles of serum exosomal miRNAs were identified between the two groups. In the first validation cohort, reverse transcription-polymerase chain reaction results indicated elevated serum exosomal miR-3174 and miR-6750-5p expressions, along with reduced miR-192-3p levels in the recurrence group compared to the non-recurrence group. Receiver operating characteristic (ROC) curves and Kaplan-Meier survival analysis demonstrated significant correlations between expressions of exosomal miR-3174 and miR-192-3p and the risk of postoperative recurrence. These findings were further validated in the second cohort, confirming the elevation of both miRNAs in the recurrence group and their associations with recurrence risk. Additionally, serum exosomal miR-3174 levels increased in recurrent cases compared to their baseline levels.
Conclusion:
Circulating exosomal microRNA signatures may influence the risk of postoperative recurrence in CRSwNP patients. Serum exosomal elevated exosomal miR-3174 and decreased miR-192-3p were correlated with CRSwNP recurrence risk.
Insights
Serum exosomal microRNAs (miRNAs) show potential for predicting chronic rhinosinusitis with nasal polyps (CRSwNP) recurrence. Elevated miR-3174 and reduced miR-192-3p levels in exosomes correlate with higher CRSwNP recurrence risk.
Area of Science:
- Biochemistry
- Genetics
- Immunology
Background:
- Exosomes are key carriers of transcripts and potential biomarkers for inflammatory diseases.
- The role of serum exosomal microRNAs (miRNAs) in chronic rhinosinusitis with nasal polyps (CRSwNP) remains unclear.
Purpose of the Study:
- To investigate the potential of serum exosomal miRNAs as biomarkers for predicting postoperative recurrence in CRSwNP patients.
- To identify specific exosomal miRNA signatures associated with CRSwNP recurrence.
Main Methods:
- Prospective exploratory cohort study with miRNA sequencing of serum exosomes from CRSwNP patients.
- Validation in two independent prospective cohorts using reverse transcription-polymerase chain reaction (RT-PCR).
- Assessment of miRNA predictive values for postoperative recurrence over a two-year follow-up period.
Main Results:
- Distinct serum exosomal miRNA profiles were identified between recurrent and non-recurrent CRSwNP groups.
- Elevated serum exosomal miR-3174 and miR-6750-5p, and reduced miR-192-3p were observed in the recurrence group.
- Significant correlations were found between exosomal miR-3174 and miR-192-3p levels and the risk of CRSwNP recurrence, confirmed in validation cohorts.
Conclusions:
- Circulating exosomal miRNA signatures can influence the risk of postoperative recurrence in CRSwNP.
- Elevated serum exosomal miR-3174 and decreased miR-192-3p are correlated with CRSwNP recurrence risk.
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