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Updated: Jun 15, 2025

Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers

Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers

Published on: August 9, 2022

Fluorogenic RNA-Based Biosensors of Small Molecules: Current Developments, Uses, and Perspectives.

Janine Kehrli¹, Claire Husser¹, Michael Ryckelynck¹

¹Université de Strasbourg, CNRS, Architecture et Réactivité de l'ARN, UPR 9002, F-67000 Strasbourg, France.

|August 28, 2024

Summary

Fluorogenic RNA-based biosensors (FRBs) offer a sensitive and selective method for detecting small molecules. This review explores aptamer modules, design strategies, and applications for FRB development.

Keywords:

FLAP RNA fluorescent reporters fluorogenic RNA-based biosensors light-up aptamer molecular imaging selection small molecule structure-switching aptamer synthetic nucleic acids

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Area of Science:

Biotechnology and Molecular Biology
Chemical Biology
Biosensor Technology

Background:

Small molecules are crucial targets for diagnostics, disease monitoring, and contaminant detection.
Existing detection methods often lack the sensitivity, specificity, or multiplexing capabilities required.
Fluorogenic RNA-based biosensors (FRBs) offer a promising alternative by integrating molecular portability with fluorescence-based detection.

Purpose of the Study:

To review the current landscape of sensing and reporting aptamer modules for FRB design.
To discuss methodologies for discovering novel aptamer specificities.
To explore functional connection strategies and applications of FRBs.

Main Methods:

Review of existing literature on aptamer selection and characterization.
Analysis of different strategies for linking sensing and reporting aptamer modules.
Compilation and discussion of reported FRB applications.

Main Results:

Identification of diverse sensing and reporting aptamer modules available for FRB construction.
Overview of established and emerging aptamer discovery techniques.
Demonstration of FRBs' utility in detecting various small molecules for diagnostic and monitoring purposes.

Conclusions:

FRBs provide a versatile platform for sensitive and selective small molecule detection.
Further improvements in FRB performance can be achieved through alternative nucleotide chemistries.
Expanded application scope is anticipated with advancements in FRB design and engineering.