Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Free-Electron Laser-Based Extended Wide-Field Mid-Infrared Photothermal Imaging for Biomedical and Microplastic Analysis.

ACS omega·2026
Same author

On-Chip Raman Spectroscopy for Rapid Antimicrobial Susceptibility Testing from Blood Cultures.

Analytical chemistry·2026
Same author

Light-guiding capillaries: a robust optofluidic platform for nanoparticle tracking analysis.

Lab on a chip·2026
Same author

From bulk samples to single cells: measurement strategies in Raman-based antibiotic susceptibility testing.

Analytical and bioanalytical chemistry·2026
Same author

Generic microfluidic platform for digital droplet-based bioassays.

RSC advances·2026
Same author

Current trends in machine learning for surface-enhanced Raman spectroscopy.

The Analyst·2026

Related Experiment Video

Updated: Jun 26, 2026

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor IRIS
11:04

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor IRIS

Published on: May 3, 2011

14.6K

Imaging Diffractometric Biosensors for Label-Free, Multi-Molecular Interaction Analysis.

Cornelia Reuter1,2, Walter Hauswald1, Sindy Burgold-Voigt1,2

  • 1Leibniz Institute of Photonic Technology, Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research (LPI), 07745 Jena, Germany.

Biosensors
|August 28, 2024
PubMed
Summary
This summary is machine-generated.

This study introduces a novel imaging diffractometric biosensor for label-free detection of biomolecules. The biosensor achieves high sensitivity, enabling simultaneous analysis of various targets like viral DNA and proteins in human plasma.

Keywords:
diffractive biosensordiffractometric imaginglabel-free interaction analysisnucleic acid detectionoptical biosensorsprotein detection

More Related Videos

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
13:57

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects

Published on: February 18, 2014

29.3K
Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
09:30

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Published on: August 6, 2018

9.4K

Related Experiment Videos

Last Updated: Jun 26, 2026

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor IRIS
11:04

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor IRIS

Published on: May 3, 2011

14.6K
Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
13:57

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects

Published on: February 18, 2014

29.3K
Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
09:30

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Published on: August 6, 2018

9.4K

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Biophysics

Background:

  • Conventional biosensors often require labeling for detection, adding complexity.
  • Existing label-free biosensors may compromise on detection sensitivity.
  • There is a need for advanced biosensing platforms offering simplicity and high performance.

Purpose of the Study:

  • To develop and validate a novel imaging diffractometric biosensor system.
  • To enable label-free detection and simultaneous analysis of diverse biomolecules.
  • To assess the biosensor's performance in detecting clinically relevant targets in complex matrices.

Main Methods:

  • Development of a diffractive biosensor chip and optical reader assembly.
  • Implementation of a label-free assay for molecular interaction analysis.
  • Application for detecting viral DNA and protein antigens directly in human plasma.

Main Results:

  • The biosensor successfully captures entire assays in a single, comprehensive image.
  • Achieved a detection limit of 4.2 pg/mm², comparable to other label-free optical biosensors.
  • Demonstrated applicability for detecting viral DNA and protein antigens in human plasma.

Conclusions:

  • The novel imaging diffractometric biosensor offers a robust and simple method for label-free biomolecule detection.
  • This technology has the potential for broad applications in molecular interaction analysis.
  • The system advances biosensing capabilities by providing simultaneous, sensitive detection without labeling.