Anti-Allergic and Anti-Inflammatory Signaling Mechanisms of Natural Compounds/Extracts in In Vitro System of RBL-2H3...

Area of Science:

• Immunology and Pharmacology
• In vitro models for allergic and inflammatory responses

Background:

• RBL-2H3 cells are crucial in allergic and immunological research.
• FCεRI signaling, including MAPK, NF-κB, and JAK/STAT pathways, is key in mast and basophil cell inflammation.

Purpose of the Study:

• To systematically review signaling pathways targeted by anti-allergic/anti-inflammatory compounds in RBL-2H3 cells.
• To identify common molecular mechanisms underlying these effects.

Main Methods:

• Systematic literature search (PubMed, Scopus, etc.) for articles post-2015.
• Risk of bias assessment using a modified CONSORT checklist for in vitro studies.
• Extraction and synthesis of data on cell lines, treatments, assays, findings, and signaling pathways.

Main Results:

• Thirty-eight articles were included in the review.
• FCεRI and downstream pathways (Lyn, Sky, PLCγ, MAPK) were frequently studied.
• The JAK/STAT pathway emerged as a potential signaling mechanism.

Conclusions:

• Anti-inflammatory compounds can modulate multiple signaling pathways in RBL-2H3 cells, beyond just MAPK.
• Further validation using human mast cells is recommended to confirm clinical relevance.