Related Experiment Video
Updated: Jun 15, 2025
A Step by Step Protocol for Subretinal Surgery in Rabbits
Published on: September 13, 2016
Intraoperative Closure of Large Full-Thickness Macular Holes with Perfluorocarbon Liquid
Francesco Sabatino1,2, Sandra Banderas-García1,2, Niall Patton1,2
1Norfolk and Norwich University Hospitals, NHS Foundation Trust, Norwich, Norfolk, United Kingdom ; and.
Purpose:
To report the role of perfluorocarbon liquid (PFCL) and passive extrusion for the management of large full-thickness macular holes (FTMHs).
Methods:
A standard pars plana vitrectomy with induction of posterior vitreous detachment was performed for all patients. After internal limiting membrane peel, a bubble of perfluorocarbon liquid (PFCL) was injected over the posterior pole, and passive extrusion of fluid was performed with a backflush instrument below the PFCL bubble, without touching the FTMH edges, until the FTMH center was reached. Intraoperative optical coherence tomography showed the formation of an inner retina roof in all cases and confirmed intraoperative FTMH closure. Complete PFCL removal was performed after fluid-air exchange, and gas tamponade was used in all cases.
Results:
Preoperative FTMH mean aperture size was 761 µ m, and SD 100 µ m (range 682-918 µ m). Full-thickness macular hole closure was achieved in all eyes and visualized intraoperatively with optical coherence tomography. After an average follow-up of 2 months, there was improvement in the mean best-corrected visual acuity and central scotoma.
Conclusion:
FTMH closure can be achieved intraoperatively with the use of PFCL and passive extrusion. The described surgical technique could be a valid alternative for the repair of large FTMHs.
