Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Finite element analysis of variable hollowness and experimental validation of optimized uniformly tapered layered hollow roller bearings.

Scientific reports·2026

Same author

Quantification of cholesterol incorporation in giant unilamellar vesicles produced by a modified cDICE method.

Soft matter·2026

Same author

Refined affinity purification protocol for CAMs using minimal mouse brain material.

Journal of neuroscience methods·2026

Same author

New perspectives on cell architectural control.

Current opinion in cell biology·2026

Same author

Mapping spatial organization of in vitro neuronal networks using high-content imaging.

Scientific reports·2025

Same author

Actin and vimentin jointly control cell viscoelasticity and compression stiffening.

Molecular biology of the cell·2025

Same journal

MT-MRI for detection of renal interstitial fibrosis in renovascular disease.

Scientific reports·2026

Same journal

Detection of underground objects from GPR data using a lightweight YOLO-based approach.

Scientific reports·2026

Same journal

Early systemic inflammatory-metabolic trajectory phenotypes are associated with survival outcomes in metastatic renal cell carcinoma treated with nivolumab.

Scientific reports·2026

Same journal

Water balance components in a dry-seeded rice-wheat system: Untangling the effects of tillage and mulching practices.

Scientific reports·2026

Same journal

Topological approaches to quantum tensor train compression via ZX-calculus and SVD.

Scientific reports·2026

Same journal

determinants of flood impacts and adaptive capacity among market vendors in Walukuba-Masese, Jinja city, Uganda.

Scientific reports·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 14, 2025

Experimental and Data Analysis Workflow for Soft Matter Nanoindentation

Experimental and Data Analysis Workflow for Soft Matter Nanoindentation

Published on: January 18, 2022

Cell spheroid viscoelasticity is deformation-dependent.

Ruben C Boot¹, Anouk van der Net², Christos Gogou²

¹Department of Chemical Engineering, Delft University of Technology, Delft, 2629, HZ, The Netherlands.

Scientific Reports

|August 28, 2024

Summary

This summary is machine-generated.

Cellular spheroids show surface tension that depends on how much they deform. This challenges the idea that surface tension always increases with applied force in biological tissues.

More Related Videos

Author Spotlight: In Vitro Hydrogel Model for Glioblastoma Microenvironment Study

Author Spotlight: In Vitro Hydrogel Model for Glioblastoma Microenvironment Study

Published on: September 22, 2023

An Efficient and Flexible Cell Aggregation Method for 3D Spheroid Production

An Efficient and Flexible Cell Aggregation Method for 3D Spheroid Production

Published on: March 27, 2017

Related Experiment Videos

Last Updated: Jun 14, 2025

Experimental and Data Analysis Workflow for Soft Matter Nanoindentation

Experimental and Data Analysis Workflow for Soft Matter Nanoindentation

Published on: January 18, 2022

Author Spotlight: In Vitro Hydrogel Model for Glioblastoma Microenvironment Study

Author Spotlight: In Vitro Hydrogel Model for Glioblastoma Microenvironment Study

Published on: September 22, 2023

An Efficient and Flexible Cell Aggregation Method for 3D Spheroid Production

An Efficient and Flexible Cell Aggregation Method for 3D Spheroid Production

Published on: March 27, 2017

Area of Science:

Biophysics
Cell Biology
Tissue Engineering

Background:

Tissue surface tension is crucial for cell sorting and fusion.
Previous studies suggest multicellular spheroids actively reinforce surface tension under force.

Purpose of the Study:

Investigate the role of force duration and spheroid deformability on tissue surface tension.
Determine if surface tension reinforcement is consistent across varying applied forces.

Main Methods:

Utilized high-throughput microfluidic micropipette aspiration.
Measured viscoelastic creep behavior of NIH3T3 and HEK293T cell spheroids.
Varied applied pressures and monitored cellular retraction dynamics.

Main Results:

Larger spheroid deformations correlated with faster cellular retraction post-pressure release.
Less deformable NIH3T3 spheroids (with higher alpha-smooth muscle actin) showed slower retraction for smaller deformations.
HEK293T spheroids exhibited retraction only at higher pressures and deformations, despite increased viscosity.

Conclusions:

Spheroid viscoelasticity is dependent on deformation.
The reinforcement of surface tension at larger aspiration pressures is questionable.
Findings challenge current models of tissue mechanics and surface tension dynamics.