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Development of a competitive ELISA based on the LSDV A33 antigen
Guohua Chen1,2,3, Xiaobing He1,2,3, Zhenzhen Gao1,2,3
1State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
Virology Journal
|August 28, 2024
Summary
A new competitive ELISA test was developed to detect Capripoxvirus antibodies. This sensitive and specific assay is crucial for diagnosing diseases like Lumpy Skin Disease in livestock.
Area of Science:
- Veterinary Virology
- Immunodiagnostics
Background:
- Capripoxviruses, including Goatpoxvirus (GTPV), Sheeppoxvirus (SPPV), and Lumpy Skin Disease Virus (LSDV), cause significant economic losses globally.
- Existing diagnostic tools for detecting antibodies against these viruses are limited in effectiveness and convenience.
Purpose of the Study:
- To establish a reliable polyclonal antibody competitive enzyme-linked immunosorbent assay (c-ELISA) for detecting serogroup-specific antibodies against Capripoxviruses.
- To validate the diagnostic performance of the developed c-ELISA.
Main Methods:
- A c-ELISA was developed using the major Lumpy Skin Disease Virus (LSDV) antigen A33.
- Serum samples (n=605) were used to optimize the assay and determine the cut-off value based on percent inhibition (PI).
- Diagnostic sensitivity (sn) and specificity (sp) were evaluated using receiver operator characteristic (ROC) analysis.
Main Results:
- The optimal cut-off value was determined to be a PI of >57.61%.
- The developed c-ELISA achieved a diagnostic sensitivity of 96.4% and a diagnostic specificity of 98.5% within a >95% confidence interval.
- The assay demonstrated high reliability for serological investigations.
Conclusions:
- The developed c-ELISA is a sensitive, specific, and reliable tool for detecting Capripoxvirus antibodies.
- This assay provides a valuable diagnostic method for serological investigations of LSDV, GTPV, and SPPV infections.
- The c-ELISA can aid in managing and controlling economically significant Capripoxvirus diseases in endemic regions.
