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The Effect of Ascorbic Acid on Hepatic Ischaemia-Reperfusion Injury in Wistar Rats: An Experimental Study
Jorge Luiz Saraiva Ximenes1,2, Joel Avancini Rocha-Filho1,2, Flavio Henrique Ferreira Galvão1,3
1Laboratório de Investigação Medica 37, Departamento de Gastroenterologia, Hospital das Clínicas HCFMUSP, Faculdade de Medicina, Universidade de São Paulo, São Paulo 05508-220, SP, Brazil.
Ascorbic acid (vitamin C) given before and during liver ischaemia-reperfusion (IR) injury in rats reduced liver damage. This timing of vitamin C administration improved liver enzymes, inflammation, and blood flow, suggesting a protective effect against IR injury.
Area of Science:
- Hepatology
- Surgical Research
- Biochemistry
Background:
- Liver ischaemia-reperfusion (IR) injury is a significant complication in hepatic surgeries.
- IR injury involves oxidative stress, immune cell activation, and release of inflammatory mediators.
- Ascorbic acid (vitamin C) possesses antioxidant properties with potential to mitigate IR injury.
Purpose of the Study:
- To evaluate the efficacy of ascorbic acid infusion at various time points during hepatic IR in a rat model.
- To determine the optimal timing for ascorbic acid administration to reduce liver injury.
Main Methods:
- Thirty-six male Wistar rats underwent hepatic IR.
- Animals were divided into control and experimental groups receiving ascorbic acid before ischaemia, before reperfusion, or before both.
- Hepatic enzymes, cytokines, and portal blood flow were measured.
Main Results:
- All animals experienced hepatic IR injury.
- Ascorbic acid administration before both ischaemia and reperfusion resulted in significantly lower liver enzyme levels.
- This group also exhibited reduced inflammation and improved portal venous flow compared to other groups.
Conclusions:
- Divided doses of ascorbic acid administered before and during hepatic IR may be beneficial in reducing liver injury.
- Timing of ascorbic acid administration is crucial for its protective effects against IR-induced liver damage.
