Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

4.0K
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
4.0K
MicroRNAs01:22

MicroRNAs

3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

One-Shot Pd(II)-Catalyzed Multiple C-H Activation Enables Modular Construction of Fluorenylidene Oxindole-Based Multi(Polycyclic) Aromatic Enes.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

Cardiovascular risks and hazards associated with deep space exploration.

Communications medicine·2026
Same author

Brain and knee joint degeneration following anterior cruciate ligament injury in a mouse model of Alzheimer's disease.

JBMR plus·2026
Same author

DFT Calculation-Assisted Virtual Screening to Refine Chiral Phosphoric Acid-Catalyzed Allylboration Enabling Organocatalytic Synthesis of Tetrafibricin C21-C40 Fragment.

JACS Au·2026
Same author

DLK1 is a GATA1s-driven dependency and therapeutic target in Down syndrome-associated myeloid leukemia.

Blood advances·2026
Same author

Cu-Catalyzed [1,3]-Fluorine Rearrangement Reactions of <i>N</i>-Fluoroanilines.

Organic letters·2026

Related Experiment Video

Updated: Jun 14, 2025

Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System
09:28

Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System

Published on: August 25, 2022

2.7K

Simulated Microgravity Alters Gene Regulation Linked to Immunity and Cardiovascular Disease.

Candice G T Tahimic1,2, Sonette Steczina2,3, Aimy Sebastian4

  • 1Department of Biology, University of North Florida, Jacksonville, FL 32224, USA.

Genes
|August 29, 2024
PubMed
Summary
This summary is machine-generated.

Simulated microgravity impacts cardiovascular health differently based on sex. Female rats showed increased oxidative damage, while males exhibited inflammatory responses, highlighting the need for sex-specific countermeasures.

Keywords:
cardiovascular systemhindlimb unloadingimmune responsemicrogravitytranscriptomics

More Related Videos

Exploring the Effects of Spaceflight on Mouse Physiology using the Open Access NASA GeneLab Platform
11:08

Exploring the Effects of Spaceflight on Mouse Physiology using the Open Access NASA GeneLab Platform

Published on: January 13, 2019

12.2K
Reduced-gravity Environment Hardware Demonstrations of a Prototype Miniaturized Flow Cytometer and Companion Microfluidic Mixing Technology
13:59

Reduced-gravity Environment Hardware Demonstrations of a Prototype Miniaturized Flow Cytometer and Companion Microfluidic Mixing Technology

Published on: November 13, 2014

13.7K

Related Experiment Videos

Last Updated: Jun 14, 2025

Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System
09:28

Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System

Published on: August 25, 2022

2.7K
Exploring the Effects of Spaceflight on Mouse Physiology using the Open Access NASA GeneLab Platform
11:08

Exploring the Effects of Spaceflight on Mouse Physiology using the Open Access NASA GeneLab Platform

Published on: January 13, 2019

12.2K
Reduced-gravity Environment Hardware Demonstrations of a Prototype Miniaturized Flow Cytometer and Companion Microfluidic Mixing Technology
13:59

Reduced-gravity Environment Hardware Demonstrations of a Prototype Miniaturized Flow Cytometer and Companion Microfluidic Mixing Technology

Published on: November 13, 2014

13.7K

Area of Science:

  • Space physiology
  • Cardiovascular research
  • Molecular biology

Background:

  • Microgravity causes fluid shifts and reduced activity, leading to cardiovascular deconditioning.
  • Extended spaceflight poses unknown risks to crew cardiovascular health.
  • The hindlimb unloading (HU) rat model simulates microgravity's effects.

Purpose of the Study:

  • To investigate cardiac responses to prolonged simulated microgravity and recovery.
  • To test the hypothesis of increased oxidative damage and altered gene expression.
  • To identify sex-dependent cardiovascular changes under simulated microgravity.

Main Methods:

  • Male and female Long-Evans rats (3 and 9 months old) underwent HU for up to 90 days.
  • Cardiac tissue was analyzed for oxidative damage marker 8-hydroxydeoxyguanosine (8-OHdG).
  • RNA sequencing (RNAseq) assessed gene expression in left ventricular tissue.

Main Results:

  • Sex-dependent changes in 8-OHdG and antioxidant gene expression were observed.
  • Younger females showed elevated 8-OHdG after 14 days HU; males did not.
  • Older males displayed upregulated pro-inflammatory and immune pathways post-HU.

Conclusions:

  • Simulated microgravity induces sex-specific cardiac alterations.
  • Oxidative stress and inflammatory pathways are key targets for countermeasures.
  • Future countermeasures must consider sex differences for cardiovascular health in space.