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Expression Pattern of PDE4B, PDE4D, and SFRP5 Markers in Colorectal Cancer
Mateo Bevanda1, Nela Kelam2,3, Anita Racetin2,3
1Department of Surgery, School of Medicine, University of Mostar, University Hospital Mostar, Bijeli Brijeg bb, 88000 Mostar, Bosnia and Herzegovina.
Medicina (Kaunas, Lithuania)
|August 29, 2024
Summary
This study investigated phosphodiesterase 4B (PDE4B), phosphodiesterase 4D (PDE4D), and secreted frizzled related protein 5 (SFRP5) in colorectal cancer (CRC). Results show these markers are underexpressed in CRC, with SFRP5 potentially serving as a promising prognostic biomarker.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Colorectal cancer (CRC) is a prevalent gastrointestinal malignancy.
- Novel diagnostic and prognostic markers are crucial for understanding CRC.
- Tumor profiling requires identification of key molecular indicators.
Purpose of the Study:
- To investigate the expression of PDE4B, PDE4D, and SFRP5 in CRC tissues.
- To analyze the correlation between marker expression and CRC stages.
- To evaluate the prognostic significance of these markers in CRC patients.
Main Methods:
- Immunohistochemistry was used to examine protein expression of PDE4B, PDE4D, and SFRP5.
- RNA-sequencing data from UCSC Xena browser analyzed gene expression levels.
- Kaplan-Meier survival analysis assessed prognostic implications.
Main Results:
- PDE4B, PDE4D, and SFRP5 showed significant differential expression in CRC tissues compared to controls.
- These genes were found to be significantly underexpressed in CRC tissues.
- Lower SFRP5 expression correlated with longer overall survival, suggesting a tumor-suppressive role.
Conclusions:
- SFRP5 is identified as a potential prognostic biomarker for colorectal cancer.
- Alterations in PDE4B, PDE4D, and SFRP5 expression are linked to CRC progression and staging.
- These findings contribute to understanding the molecular mechanisms in CRC development.
