Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

Mismatch Repair

Mismatch Repair

Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

What is Genetic Engineering?

What is Genetic Engineering?

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Engineered prime editors with minimal genomic errors.

Nature·2025

Same author

Mutant p53 exploits enhancers to elevate immunosuppressive chemokine expression and impair immune checkpoint inhibitors in pancreatic cancer.

Immunity·2025

Same author

RNA Dynamics Regulate Transcriptional Condensate Vivacity to Drive Gene Coordination.

bioRxiv : the preprint server for biology·2025

Same author

Mutant p53 Exploits Enhancers to Elevate Immunosuppressive Chemokine Expression and Impair Immune Checkpoint Inhibitors in Pancreatic Cancer.

bioRxiv : the preprint server for biology·2024

Same author

The future of open research policy should be evidence based.

Proceedings of the National Academy of Sciences of the United States of America·2024

Same author

Single-cell nascent RNA sequencing unveils coordinated global transcription.

Nature·2024

Same journal

Genetic Impacts on Variability of Body Fat Distribution Uncover Gene-Environment and Gene-Gene Interactions.

bioRxiv : the preprint server for biology·2026

Same journal

16S ribosomal RNA modification drives transcript-specific translation efficiency.

bioRxiv : the preprint server for biology·2026

Same journal

FlcE latches onto the FliL-stator complex to turbocharge flagellar motility in <i>Borrelia burgdorferi</i>.

bioRxiv : the preprint server for biology·2026

Same journal

Synaptic pruning, myelination and the emergence of psychiatric disorders in late adolescence.

bioRxiv : the preprint server for biology·2026

Same journal

Structural and functional insights into the Rcs phosphorelay.

bioRxiv : the preprint server for biology·2026

Same journal

The structural basis of RanGAP1 regulation and catalysis in nuclear transport.

bioRxiv : the preprint server for biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 14, 2025

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

Published on: February 17, 2023

Engineered prime editors with minimal genomic errors.

Vikash P Chauhan, Phillip A Sharp, Robert Langer

Biorxiv : the Preprint Server for Biology

|August 30, 2024

Summary

This summary is machine-generated.

New prime editors (PEs) nearly eliminate genome editing errors by repositioning DNA breaks. This strategy significantly reduces unwanted insertions and deletions (indels), improving the precision of gene editing technologies.

More Related Videos

Mouse Genome Engineering Using Designer Nucleases

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

Related Experiment Videos

Last Updated: Jun 14, 2025

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

Published on: February 17, 2023

Mouse Genome Engineering Using Designer Nucleases

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

Area of Science:

Molecular Biology
Genetics
Biotechnology

Background:

Prime editors (PEs) are advanced gene editing tools capable of precise DNA modifications.
A key challenge in PE technology is the occurrence of insertion and deletion (indel) errors, which reduce editing efficiency and specificity.
These errors arise from a bias favoring the retention of competing DNA strands during the editing process.

Purpose of the Study:

To engineer a novel prime editor with significantly reduced indel errors.
To enhance the efficiency and accuracy of programmed genome modifications.
To improve the overall utility of prime editing for therapeutic and research applications.

Main Methods:

Rational design of the Cas9-nickase component within prime editors to alter DNA nick positioning.
Investigating the destabilization of competing 5' DNA strands to favor edited 3' strands.
Integrating error-suppressing strategies with advanced efficiency-boosting architectures to create next-generation prime editors.

Main Results:

Repositioning prime editor nicks effectively destabilizes competing 5' DNA strands.
Engineered prime editors demonstrate strikingly low indel error rates.
The next-generation prime editor (vPE) achieves comparable efficiency to previous editors but with up to 60-fold lower indel errors.
vPE enables high edit:indel ratios, reaching as high as 465:1.

Conclusions:

Repositioning DNA breaks in prime editors is a potent strategy for minimizing indel errors.
The developed vPE represents a significant advancement in prime editing technology, offering enhanced precision.
This improved accuracy in genome editing holds promise for safer and more effective gene-based therapies and research.