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Novel Honeycomb Nanoclay Frameworks With Hemostatic and Antibacterial Properties
Mélyssa Cambronel1, Kan Wongkamhaeng2, Christelle Blavignac3
1Université Clermont Auvergne, Clermont Auvergne INP, CNRS, Clermont-Ferrand, France.
Summary
Researchers developed novel antibacterial topical hemostatic agents (THAs) by combining Nanoclay Microsphere Frameworks (NMFs) with silver nanoparticles (AgNPs). These AgNP-loaded NMFs enhance clot strength and inhibit bacterial growth, offering a promising solution for wound management.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Materials Chemistry
Background:
- Nanoclay Microsphere Frameworks (NMFs) are high surface area absorbents that promote rapid hemostasis.
- The need for effective topical hemostatic agents (THAs) with antimicrobial properties is critical for wound management.
- Silver nanoparticles (AgNPs) possess well-documented antimicrobial capabilities.
Purpose of the Study:
- To develop novel antibacterial topical hemostatic agents (THAs) by integrating silver nanoparticles (AgNPs) onto Nanoclay Microsphere Frameworks (NMFs).
- To evaluate the hemostatic efficacy, silver nanoparticle stability, release kinetics, and antimicrobial activity of the developed AgNP-loaded NMFs.
- To assess the cytotoxicity of the novel AgNP-loaded NMFs.
Main Methods:
- AgNPs were anchored onto NMFs using a chemical co-reduction approach followed by freeze-processing.
- Hemostatic properties were assessed by measuring clot strength and fibrin fiber diameter.
- Antimicrobial activity was evaluated against Pseudomonas aeruginosa and Staphylococcus aureus.
- Cytotoxicity was determined by measuring cell mortality at varying AgNP concentrations.
Main Results:
- Silver-loaded NMFs maintained their unique architecture and demonstrated a 55% increase in clot strength compared to controls.
- A significant decrease in mean fibrin fiber diameter was observed.
- Effective inhibition of Pseudomonas aeruginosa and Staphylococcus aureus growth was achieved at 22 and 30 ppm released silver, respectively.
- Low cytotoxicity was confirmed, with cell mortality ranging from 6.3% to 9.1%.
Conclusions:
- Silver-loaded NMFs represent a promising new class of topical hemostatic agents with dual hemostatic and antimicrobial functions.
- The developed AgNP-NMFs offer enhanced hemostatic properties and effective control of bacterial infections.
- These agents show potential for secondary management of hemostasis in infected wounds.
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