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Related Experiment Video

Updated: Jun 14, 2025

Micron-scale Phenotyping Techniques of Maize Vascular Bundles Based on X-ray Microcomputed Tomography

Micron-scale Phenotyping Techniques of Maize Vascular Bundles Based on X-ray Microcomputed Tomography

Published on: October 9, 2018

Root Anatomy: Preparing, Imaging, and Analyzing Maize Root Cross-Sections.

Jagdeep Singh Sidhu¹, Hannah M Schneider²

¹Department of Plant Science, Pennsylvania State University, University Park, Pennsylvania 16802, USA.

Cold Spring Harbor Protocols

|August 30, 2024

Summary

This study presents a standardized protocol for analyzing maize root anatomy. This method aids in understanding plant stress tolerance and developing more efficient crop varieties.

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A Simple Protocol for Mapping the Plant Root System Architecture Traits

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Last Updated: Jun 14, 2025

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A Simple Protocol for Mapping the Plant Root System Architecture Traits

A Simple Protocol for Mapping the Plant Root System Architecture Traits

Published on: February 10, 2023

Area of Science:

Plant Biology
Agronomy
Genetics

Background:

Root anatomy is crucial for maize growth, nutrient uptake, and stress tolerance.
Understanding root traits can improve crop efficiency and stress resilience.
Characterizing root anatomy is key to leveraging these traits.

Purpose of the Study:

To provide a systematic and accessible protocol for maize root cross-section analysis.
To enable accurate visualization and quantification of root anatomical traits.
To support phenotyping studies for crop improvement.

Main Methods:

Detailed steps for root sectioning (manual or vibratome) and slide preparation.
Toluidine blue staining for enhanced visualization of root tissues.
Light microscopy imaging and both manual and semi-automated image analysis for trait extraction.

Main Results:

A comprehensive protocol for preparing, imaging, and analyzing maize root cross-sections.
Enables visualization and quantification of diverse anatomical features.
Demonstrates simplicity, adaptability, and accessibility for various study scales.

Conclusions:

The standardized protocol facilitates in-depth analysis of maize root structures.
Essential for understanding plant development and adaptive strategies for stress tolerance.
Applicable to maize and other plant species for phenotyping and breeding efforts.