Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Human antisperm monoclonal antibodies constructed postvasectomy.

J C Herr, J E Fowler, S S Howards

Biology of Reproduction

|April 1, 1985

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A lateral-to-mesial organization of human ventral visual cortex at birth.

Brain structure & function·2018

Same author

Parent coaching increases the parents' capacity for reflection and self-evaluation: results from a clinical trial in autism.

Attachment & human development·2018

Same author

High-quality human and rat spermatozoal RNA isolation for functional genomic studies.

Andrology·2018

Same author

Information Technology Measurement and Testing Activities at NIST.

Journal of research of the National Institute of Standards and Technology·2016

Same author

Intra- and inter-individual differences in human sperm DNA methylation.

Andrology·2016

Same author

Sperm Lysozyme-Like Protein 1 (SLLP1), an intra-acrosomal oolemmal-binding sperm protein, reveals filamentous organization in protein crystal form.

Andrology·2015

Researchers successfully created human monoclonal antibodies targeting sperm antigens following vasectomy. This breakthrough utilized somatic cell hybridization to capture autoantibodies from the post-vasectomy autoimmune state.

Area of Science:

Immunology
Reproductive Biology

Background:

Vasectomy can trigger an autoimmune response against sperm and spermatogenic cell antigens due to barrier disruption.
Antisperm antibodies are detectable in serum and associated with the post-vasectomy autoimmune state.

Purpose of the Study:

To develop a method for capturing and producing human monoclonal antisperm autoantibodies from individuals post-vasectomy.
To characterize the reactivity of these antibodies against sperm antigens.

Main Methods:

Sera and peripheral blood lymphocytes were collected 6-9 months after vasectomy.
Lymphocyte-myeloma hybridomas were generated by fusing patient lymphocytes with a mouse myeloma cell line.
Hybridomas secreting immunoglobulin were selected and screened for antisperm activity using ELISA.

Keywords:

Antibodies Autoantibodies Autoimmune Response Biology Family Planning Hematological Effects Immunity Immunoglobulin Alterations Immunologic Factors Immunological Effects Male Sterilization Physiology Sterilization, Sexual Vasectomy

Related Experiment Videos

Main Results:

Eight mouse-human fusions produced 205 hybridomas secreting human monoclonal antibodies (mAbs).
Eleven of these mAbs demonstrated antisperm reactivity.
Two specific hybridomas, HAS-1 (IgM, kappa) and HAS-2 (IgM, lambda), were detailed, recognizing epitopes on the sperm midpiece and tail, respectively.

Conclusions:

Somatic cell hybridization is an effective technique for capturing human antisperm autoantibodies from the post-vasectomy autoimmune state.
This method yields monoclonal antibodies with specific reactivity to sperm antigens, potentially useful for further research.