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Related Concept Videos

Genome Annotation and Assembly03:36

Genome Annotation and Assembly

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The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
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Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.
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Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
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Updated: Jun 14, 2025

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
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Asteraceae genome database: a comprehensive platform for Asteraceae genomics.

Liang Wang1,2, Hanting Yang3, Guoqing Xu3

  • 1State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.

Frontiers in Plant Science
|September 3, 2024
PubMed
Summary
This summary is machine-generated.

The Asteraceae Genomics Database (AGD) offers a centralized resource for analyzing diverse Asteraceae plant genomes. This platform aids researchers in gene annotation, breeding, and understanding evolutionary biology for this important plant family.

Keywords:
AsteraceaeAsteraceae Genome Database (AGD)analysis toolsgenometaxonomy

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Area of Science:

  • Plant genomics
  • Bioinformatics
  • Biodiversity conservation

Background:

  • Asteraceae, the largest angiosperm family, possesses significant medicinal and horticultural value.
  • Intricate genetic backgrounds of Asteraceae species pose challenges for genomic research.
  • Advancements in sequencing technology have generated substantial genomic data for Asteraceae.

Purpose of the Study:

  • To develop a comprehensive, centralized genomic resource for Asteraceae species.
  • To facilitate gene annotation, gene family analysis, and evolutionary studies.
  • To support genetic breeding and biodiversity conservation efforts within the Asteraceae family.

Main Methods:

  • Development of the Asteraceae Genomics Database (AGD).
  • Integration of high-quality genomic and organelle genome data.
  • Inclusion of analytical tools such as BLAST, JBrowse, and CRISPRCasFinder.

Main Results:

  • AGD provides a systematic platform for Asteraceae genomic research.
  • The database enables convenient querying, analysis, and comparison of genomic information.
  • A suite of bioinformatics tools is integrated for diverse analytical needs.

Conclusions:

  • AGD is a significant resource for advancing Asteraceae genomics.
  • The database empowers researchers in gene annotation, evolutionary biology, and genetic breeding.
  • AGD contributes to the promotion of genetic breeding and biodiversity safeguarding.