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Updated: Aug 1, 2026

Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging
Published on: April 16, 2014
Spatiotemporal dynamics of microscopic biological barrier visualized by electric-double-layer modulation imaging
Jun Kurosu1, Takato Sakamaki1, Kaname Kanai2
1Research Institute for Applied Electronics and Photonics, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, 305-8565, Japan; Department of Physics and Astronomy, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, 278-8510, Japan.
Abstract:
Live-cell label-free imaging of a microscopic biological barrier, generally referred to as 'tight junction', was realized by a recently developed electric-double-layer modulation imaging (EDLMI). The method allowed quantitative imaging of barrier integrity in real time, thus being an upper compatible of transepithelial electrical resistance (TEER) which is a conventional standard technique to evaluate spatially averaged barrier integrity. We demonstrate that the quantitative and real-time imaging capability of EDLMI unveils fundamental dynamics of biological barrier, some of which are totally different from conventional understandings.
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