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Updated: Jul 12, 2026

A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation
Published on: April 2, 2012
Progression of herpesvirus infection is inhibited by calcium reporter
Kari Kunnas1, Maija Vihinen-Ranta1, Simon Leclerc1
1Department of Biological and Environmental Science and Nanoscience Center, University of Jyväskylä, Jyvaskyla, Central Finland, Finland.
Abstract:
During infection, Herpes simplex virus type 1 (HSV-1) alters the mitochondrial structure and function of the host cell. Live-cell imaging with fluorescent reporters revealed increased mitochondrial calcium and a transient ROS enrichment after HSV-1 infection. Notably, cells co-transfected with a calcium reporter displayed smaller viral replication compartments, while those with a ROS reporter exhibited average growth of viral replication compartments. Our findings suggest that the virus-induced increase in mitochondrial calcium, followed by an increased amount of bound calcium reporter, interferes with the progression of the infection.
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