Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Disassembly of Intermediate Filaments

Disassembly of Intermediate Filaments

Intermediate filaments (IFs) do not undergo spontaneous disassembly. Enzymes, kinases, and phosphatases add and remove phosphates from specific sites to regulate their disassembly. The IF concentration in the cytoplasm also regulates the disassembly. If the concentration crosses a threshold, it activates the protein kinases in the vicinity, allowing the phosphorylation of IFs.
Keratin proteins, found at the cell periphery near cell junctions, undergo a cycle of assembly and disassembly. In Type...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Antibody-trapping presents a widespread pitfall for microscopy and genomics in the nucleus.

Nucleic acids research·2026

Same author

CLASH (Chromatin Loop Across-sample Score Harmonizer) quantifies the relative contributions of genetic variation, methylation, and CTCF occupancy on chromatin loop strength across individuals.

bioRxiv : the preprint server for biology·2026

Same author

Cohesin bridging as a physical principle of enhancer-promoter communication.

bioRxiv : the preprint server for biology·2026

Same author

The ChIP-FRiP pipeline quantifies co-binding and reveals how antibody background contributes to cohesin ChIP-seq patterns.

bioRxiv : the preprint server for biology·2026

Same author

Sequence design for three-dimensional genome folding using Akita Semifreddo.

bioRxiv : the preprint server for biology·2026

Same author

Structural variants in human congenital heart disease disrupt distal genomic regulatory contacts of developmental genes.

bioRxiv : the preprint server for biology·2026

Search research articles

Related Experiment Video

Updated: May 7, 2026

Molecular Entanglement and Electrospinnability of Biopolymers

Molecular Entanglement and Electrospinnability of Biopolymers

Published on: September 3, 2014

The physical chemistry of interphase loop extrusion.

Maxime M C Tortora¹, Geoffrey Fudenberg¹

¹Department of Quantitative and Computational Biology, University of Southern California, Los Angeles, USA.

Biorxiv : the Preprint Server for Biology

|September 4, 2024

Summary

A new model explains how cohesin regulators control genome folding via loop extrusion. This framework links molecular dynamics to large-scale chromatin architecture, revealing how protein abundance impacts genome organization.

More Related Videos

Film Extrusion of Crambe abyssinica/Wheat Gluten Blends

Film Extrusion of Crambe abyssinica/Wheat Gluten Blends

Published on: January 17, 2017

Drawing and Hydrophobicity-patterning Long Polydimethylsiloxane Silicone Filaments

Drawing and Hydrophobicity-patterning Long Polydimethylsiloxane Silicone Filaments

Published on: January 7, 2019

Related Experiment Videos

Last Updated: May 7, 2026

Molecular Entanglement and Electrospinnability of Biopolymers

Molecular Entanglement and Electrospinnability of Biopolymers

Published on: September 3, 2014

Film Extrusion of Crambe abyssinica/Wheat Gluten Blends

Film Extrusion of Crambe abyssinica/Wheat Gluten Blends

Published on: January 17, 2017

Drawing and Hydrophobicity-patterning Long Polydimethylsiloxane Silicone Filaments

Drawing and Hydrophobicity-patterning Long Polydimethylsiloxane Silicone Filaments

Published on: January 7, 2019

Area of Science:

Genomics and Molecular Biology
Biophysics
Computational Biology

Background:

Loop extrusion by structural maintenance of chromosomes (SMC) complexes organizes genomes.
In mammals, cohesin mediates loop extrusion, with dynamics influenced by accessory proteins.
The precise mechanisms by which cohesin regulators modulate genome folding are not fully understood.

Purpose of the Study:

To develop a theoretical model of cohesin loop extrusion based on first principles.
To elucidate how cohesin regulators and their interactions influence genome folding dynamics.
To connect molecular-scale motor activity to genome-wide structural consequences.

Main Methods:

Derived a model from in vivo measurements of cohesin regulator abundance and dynamics.
Systematically evaluated chemical reaction networks for cohesin-chromatin interactions.
Embedded the biochemical reaction network within biophysical chromatin simulations.

Main Results:

Identified a minimal biochemical reaction cycle consistent with experimental data.
Demonstrated how regulator roles and kinetic control emerge from the network structure.
Showed that variations in regulator abundance alter chromatin architecture across scales.

Conclusions:

The proposed model provides a quantitative framework for understanding cohesin-mediated genome folding.
Cohesin regulator dynamics play a critical role in modulating loop extrusion kinetics.
The theoretical framework bridges the gap between molecular extrusion and genome-wide organization.