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Andrographolide demonstrates anti-proliferative activity in oral cancer by promoting apoptosis, the programmed cell

Gauri Mansinh Kumbhar1, Amol Dilip Jadhav2, Supriya Kheur1

  • 1Dr. D. Y. Patil Dental College & Hospital Dr. D. Y. Patil Vidyapeeth, Sant Tukaram Nagar, Pimpri, Pune. Maharashtra. India Pune: 411018.

Iranian Journal of Basic Medical Sciences
|September 4, 2024
PubMed
Abstract

Insights

Andrographolide demonstrates anticancer potential against oral cancer cells, showing anti-proliferative and anti-migratory effects. This natural compound may serve as a future chemotherapeutic agent for oral cancer treatment.

Area of Science:

  • Pharmacology and Molecular Biology
  • Oncology
  • Bioinformatics

Background:

  • Limited research exists on Andrographolide's efficacy against oral cancer.
  • Andrographolide is a natural compound with potential anticancer properties.

Purpose of the Study:

  • To evaluate the anticancer potential of Andrographolide on oral cancer cells.
  • To utilize in-silico network analysis and in vitro assays to assess Andrographolide's effects.

Main Methods:

  • In-silico analysis included drug-likeness, target prediction, PPI networks, hub gene identification, KEGG pathway analysis, GO enrichment, and molecular docking.
  • In vitro assays involved MTT, apoptosis, cell cycle, ROS, mitochondrial membrane potential, migration assays, and gene expression analysis via PCR.

Main Results:

  • Fifteen common genes linked to proliferation, apoptosis, migration, and cell cycle regulation were identified.
  • The MAPK signaling pathway, involving MAPK1, MAPK8, MAPK14, and IL6, emerged as a key pathway.
  • In vitro results indicated anti-proliferative, anti-migratory effects, and induction of late apoptosis.

Conclusions:

  • Andrographolide exhibits significant anticancer properties against oral cancer cells.
  • The study suggests Andrographolide's potential as a chemotherapeutic drug for oral cancer.
  • The in-silico approach provides a model for future cancer treatment research.
Keywords:
Andrographolide ApoptosisCell cycleGene expressionIn-silico analysisMitochondrial membrane-Oral cancerpotential

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