Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fibril-associated Collagen01:11

Fibril-associated Collagen

2.5K
Fibril-associated collagens are a type of collagens present in the extracellular matrix with interrupted triple helices or FACIT (Fibril-associated collagens interrupted triple-helices). FACIT help connect and attach the collagen fibrils with each other as well as with other proteins of the extracellular matrix.
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
2.5K
Phases of Wound Repair01:28

Phases of Wound Repair

5.9K
Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
5.9K
Structural Protein Function01:56

Structural Protein Function

27.5K
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity.  In bones and teeth, it mineralizes to...
27.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recurrent and metastatic osteoclast-like giant cell tumor of the liver revealed by FDG PET/CT.

Clinical nuclear medicine·2012
Same author

Case-control study of single nucleotide polymorphisms of PSCA and MUC1 genes with gastric cancer in a Chinese.

Asian Pacific journal of cancer prevention : APJCP·2012
Same author

Significance of Aspergillus spp. isolation from lower respiratory tract samples for the diagnosis and prognosis of invasive pulmonary aspergillosis in chronic obstructive pulmonary disease.

Chinese medical journal·2012
Same author

Stage-specific gender differences in cognitive and neuropsychiatric manifestations of vascular dementia.

American journal of Alzheimer's disease and other dementias·2012
Same author

Oncolytic virus-mediated tumor radiosensitization in mice through DNA-PKcs-specific shRNA.

Translational cancer research·2012
Same author

A label-free electrochemiluminescence aptasensor for thrombin based on novel assembly strategy of oligonucleotide and luminol functionalized gold nanoparticles.

Biosensors & bioelectronics·2012
Same journal

Apolipoprotein C3 Promotes Retinal Angiogenesis via the MYC Pathway in Hypoxia.

Current eye research·2026
Same journal

Possible Causal Association Between Thyroid-Related Traits and Diabetic Retinopathy Risk: Evidence From 23 Medication-Taking Traits.

Current eye research·2026
Same journal

Linking Microstructure to Mechanics in ICG-Stained Lens Capsules: Insights from Nanoindentation to Electron Microscopy.

Current eye research·2026
Same journal

Corneal Sensitivity, Sub-Basal Corneal Nerve Plexus, and Tear Neuropeptides in Symptomatic Versus Asymptomatic Visual Display Terminal Users.

Current eye research·2026
Same journal

Selective Laser Trabeculoplasty Mitigates Dexamethasone-Induced Myofibroblast-Like Transformation and Dysfunction in Human Trabecular Meshwork Cells.

Current eye research·2026
Same journal

Usefulness of the Entropy of the Bayesian Posterior Distribution of the Threshold to Assess the Reliability of Measured Visual Field.

Current eye research·2026
See all related articles

Related Experiment Video

Updated: Jun 14, 2025

In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding
08:20

In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding

Published on: May 1, 2020

6.7K

A Decrease in Autophagy Increases the Level of Collagen Type I Expression in Scleral Fibroblasts.

Yingjie Zhang1, Yi Zhu2,3, Fang Li4,5

  • 1Department of Ophthalmology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.

Current Eye Research
|September 4, 2024
PubMed
Summary
This summary is machine-generated.

Autophagy inhibition in scleral fibroblasts increases collagen synthesis, offering insights into myopia development and extracellular matrix remodeling. This study reveals a key mechanism linking autophagy to collagen production in myopia.

Keywords:
COL1A1Scleral fibroblastsautophagychloroquinemyopia

More Related Videos

Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells ASCs
08:52

Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells ASCs

Published on: August 11, 2016

6.9K
Scleral Cross-linking Using Riboflavin and Ultraviolet-A Radiation for Prevention of Axial Myopia in a Rabbit Model
05:56

Scleral Cross-linking Using Riboflavin and Ultraviolet-A Radiation for Prevention of Axial Myopia in a Rabbit Model

Published on: April 3, 2016

8.9K

Related Experiment Videos

Last Updated: Jun 14, 2025

In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding
08:20

In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding

Published on: May 1, 2020

6.7K
Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells ASCs
08:52

Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells ASCs

Published on: August 11, 2016

6.9K
Scleral Cross-linking Using Riboflavin and Ultraviolet-A Radiation for Prevention of Axial Myopia in a Rabbit Model
05:56

Scleral Cross-linking Using Riboflavin and Ultraviolet-A Radiation for Prevention of Axial Myopia in a Rabbit Model

Published on: April 3, 2016

8.9K

Area of Science:

  • Ophthalmology
  • Cell Biology
  • Biochemistry

Background:

  • Autophagy dysregulation influences extracellular matrix remodeling, affecting collagen levels and protease secretion.
  • The specific role of autophagy in scleral extracellular matrix remodeling during myopia progression remains unclear.

Purpose of the Study:

  • To investigate the effect of autophagy on scleral extracellular matrix remodeling in myopia.
  • To measure autophagy levels in the sclera of myopic guinea pigs.
  • To correlate autophagy levels in human scleral fibroblasts with COL1A1 synthesis.

Main Methods:

  • Assessed COL1A1 expression and autophagy markers in guinea pig sclera using a form deprivation myopia model.
  • Utilized rapamycin and chloroquine to modulate autophagy in cultured human scleral fibroblasts.
  • Analyzed COL1A1 gene and protein expression via qPCR, Western blotting, and immunofluorescence; assessed autophagy proteins via Western blotting.

Main Results:

  • Form deprivation myopia in guinea pigs showed reduced COL1A1 expression and elevated autophagy in sclera.
  • Inhibition of autophagy in human scleral fibroblasts using chloroquine led to increased COL1A1 expression.

Conclusions:

  • Inhibiting autophagy in scleral fibroblasts enhances COL1A1 expression at both gene and protein levels.
  • This study elucidates the impact of autophagy on collagen synthesis by scleral fibroblasts, providing a mechanistic link to myopia.