A colour sensor integrated into a microcontroller platform as a reliable tool for measuring pH changes in
Ondřej Keresteš1, Miroslav Pohanka1
1Department of Molecular Pathology and Biology, Military Faculty of Medicine, University of Defence, Hradec Kralove, Czech Republic. ondrej.kerestes@gmail.com.
Analytical Methods : Advancing Methods and Applications
|September 4, 2024
Summary
A new, affordable colorimetric assay using a TCS34725 colour sensor offers a simple method for optical detection. This assay provides a significantly larger signal range than digital image colorimetry for enzyme-based detection, making it practical for point-of-care testing.
Area of Science:
- Analytical Chemistry
- Sensor Technology
- Biomedical Engineering
Background:
- Colorimetry is crucial for optical detection in point-of-care testing (POCT) and on-site analysis.
- Current methods often analyze samples sequentially, limiting throughput.
- Multiplex analysis of colored solutions is an area for improvement.
Purpose of the Study:
- To develop a simple, affordable, and effective colorimetric assay for enhanced optical detection.
- To integrate a TCS34725 color sensor and RGB LED into a 3D-printed frame for a portable colorimeter.
- To compare the performance of the developed colorimeter against digital image colorimetry (DIC).
Main Methods:
- A colorimetric assay was designed using a TCS34725 color sensor and RGB LED module.
- The components were integrated into a 3D-printed frame, creating a custom colorimeter.
- The assay was tested with model enzymes (cholinesterase, urease) that induce pH changes.
Main Results:
- The developed colorimetric assay demonstrated successful transfer to a color sensing platform.
- The custom colorimeter achieved a signal range over 200 times greater than DIC for the same samples.
- The assay showed high sensitivity with enzyme-induced pH changes.
Conclusions:
- The developed colorimetric assay is a practical and effective tool for optical detection.
- The custom colorimeter offers superior performance compared to DIC, especially for enzyme-based assays.
- This technology holds promise for advancing point-of-care and on-site diagnostic applications.
