HPLC METHOD FOR THE QUANTIFICATION OF SOME ACTIVE FLAVONOIDS IN ETHYL ACETATE EXTRACT OF LEAVES OF BUTEA MONOSPERMA...

Area of Science:

• Phytochemistry
• Pharmacognosy

Background:

• Butea monosperma (Lam.) Kuntze is a plant with traditional medicinal uses.
• Flavonoids and phenolic compounds are known for their diverse biological activities.

Purpose of the Study:

• To perform phytochemical screening of Butea monosperma leaf extracts.
• To evaluate the ethyl acetate extract for active flavonoids using High-Performance Liquid Chromatography (HPLC).

Main Methods:

• Sequential extraction of Butea monosperma leaves using solvents of increasing polarity (petroleum ether, chloroform, ethyl acetate).
• Qualitative phytochemical screening for various chemical constituents.
• HPLC analysis of the ethyl acetate extract using standard phenolic markers.

Main Results:

• Preliminary phytochemical analysis revealed the presence of steroids, triterpenoids, fatty acids, and alkaloids in the leaves.
• The ethyl acetate extract was rich in therapeutically important phytoconstituents, including steroids, triterpenoids, saponins, flavonoids, and tannins.
• Various flavonoids and phenolic compounds were detected in the ethyl acetate extract.

Conclusions:

• The study provides a preliminary report on the phytochemical profile and HPLC evaluation of Butea monosperma leaf ethyl acetate extract.
• Further research, including isolation and characterization of individual compounds, is necessary to understand the bioactive properties and potential synergies.