REMEDIAL INTERVENTION OF FERTILITY AGENT AND GENE 35 ON INDUCED CYSTIC OVARY IN RATS

Infertility is a condition with a negative health state and cystic ovary disorder is one of it is causes. PCOS is a prevalent derange till now. Therefore, current study is designed to estimate the role of Gene 35 and fertility supplements (composed from chasteberry, green tea extracts and L-arginine along with some elements),as alternative therapy for induced cystic ovary with letrozole in rats. Thirty female rats were divided erratically into four groups: G1 negative control, G2 positive group, G3 fertility group, G4 Gene 35 group and G5 combination of both Gene 35 with fertility. Vaginal smear examination was done to ensure the occurrence of PCOS. Blood samples were obtained, and sera were separated for hormonal evaluation of luteinizing hormone (LH), testosterone (T), anti mullerian hormone (AMH), follicle-stimulating hormone (FSH), prolactin (PRL) as well as insulin and ovary samples were obtained for histopathological study. Current study reveals a significant (p<0.05) increase in serum AMH, FT, LH, PRL as well as insulin and significant (p<0.05) decrease in serum FSH in PCOS groups when compared to treated groups. However, treated groups experienced significant (p<0.05) decrease in serum AMH, FT, LH, PRL with insulin and significant (p<0.05) increase in in serum FSH in comparison to positive control. Histopathological study showed marked reduction of cystic follicle along with corpora lutea predominance that reflect a good response as compared to PCOS group. From the current result, there is a noticeable improvement in number of ovarian cysts and hormones levels. Therefore, it is recommended that Gene 35 and fertility supplement can be used in the treatment of PCOS patients as supporting and treatment.