Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Complexometric Titration: Overview00:39

Complexometric Titration: Overview

6.2K
Complexometric titration involves the formation of a complex by reacting a metal ion with one or more ligands. A visual indicator often detects the end point of a complexometric titration. It is added to the metal solution before the titration, forming a stable metal–indicator complex and imparting color to the solution. As the titration approaches the equivalence point, the excess of the added ligand displaces the indicator from the metal–indicator complex, releasing the free...
6.2K
Complexometric Titration: Ligands00:43

Complexometric Titration: Ligands

930
Different monodentate and polydentate ligands are used as complexing agents in complexometric titration reactions. The formation of complexes by mono- and bidentate ligands involves two or more intermediate steps, limiting their use as complexing agents. In comparison, polydentate ligands can form complexes with metal ions in a single-step process, facilitating sharper end points. This means polydentate ligands, such as amino carboxylic acid derivatives, are most commonly employed in...
930
Colors and Magnetism03:02

Colors and Magnetism

11.6K
Color in Coordination Complexes
When atoms or molecules absorb light at the proper frequency, their electrons are excited to higher-energy orbitals. For many main group atoms and molecules, the absorbed photons are in the ultraviolet range of the electromagnetic spectrum, which cannot be detected by the human eye. For coordination compounds, the energy difference between the d orbitals often allows photons in the visible range to be absorbed and emitted, which is seen as colors by the human...
11.6K
EDTA: Direct, Back-, and Displacement Titration01:30

EDTA: Direct, Back-, and Displacement Titration

2.6K
The EDTA titration types for metal ion analysis include direct titration, back-titration, and replacement titration.
Direct titration involves buffering the metal ion solution to the desired pH and directly titrating with standard EDTA until the endpoint. The optimum pH ensures a large conditional formation constant of metal−EDTA and visibility of the free indicator color in the solution. In addition, auxiliary complexing reagents are used to prevent the precipitation of metal hydroxides...
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sphingosine-1-phosphate mitigates radiation-induced ovarian injury in rats by suppressing ferroptosis via activating the Nrf2-GCLC-GPX4 axis.

Journal of ovarian research·2026
Same author

Association between ASPECTS regions and clinical outcomes following endovascular therapy in patients with large infarcts: a post hoc analysis of the ANGEL-ASPECT trial.

Stroke and vascular neurology·2026
Same author

Construction and validation of a machine learning model for predicting early pregnancy in patients with polycystic ovary syndrome: a retrospective cohort study.

Journal of ovarian research·2026
Same author

"Internet+" Case-Based Learning Improves Perceived Learning Gains and Teaching Satisfaction in an Integrated Medical Curriculum: A Comparative Study.

Journal of medical education and curricular development·2026
Same author

Effect of Chloride Ion Concentration on the Corrosion Behavior of Mg-Gd-Y Alloys: An Experimental and First-Principles Calculation Study.

Inorganic chemistry·2026
Same author

Extracellular Vesicles as Immunomodulatory Mediators in Angiogenic-Osteogenic Coupling: Mechanistic Insights and Therapeutic Potential.

Advanced healthcare materials·2026

Related Experiment Video

Updated: Jun 14, 2025

Accumulation and Analysis of Cuprous Ions in a Copper Sulfate Plating Solution
07:00

Accumulation and Analysis of Cuprous Ions in a Copper Sulfate Plating Solution

Published on: March 20, 2019

15.0K

In Situ Formed 3,3',5,5'-Tetramethylbenzidine-Cu(I/II) Charge-Transfer Complex Intermediates Promoting Colorimetric

Jiahui Zhao1, Yixuan Zhao1, Xuefeng Shao1

  • 1School of Chemical Engineering, State Province Joint Engineering Laboratory of Zeolite Membrane Materials, College of Chemical Engineering, Jiangxi Normal University, Nanchang 330022, China.

Inorganic Chemistry
|September 4, 2024
PubMed
Summary
This summary is machine-generated.

A new colorimetric assay uses a synergistic Cu2+/Cr6+ strategy to detect Cr6+. This method accelerates 3,3

More Related Videos

Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides
11:04

Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides

Published on: September 7, 2019

9.1K
Quantifying the Binding Interactions Between CuII and Peptide Residues in the Presence and Absence of Chromophores
11:38

Quantifying the Binding Interactions Between CuII and Peptide Residues in the Presence and Absence of Chromophores

Published on: April 5, 2022

2.4K

Related Experiment Videos

Last Updated: Jun 14, 2025

Accumulation and Analysis of Cuprous Ions in a Copper Sulfate Plating Solution
07:00

Accumulation and Analysis of Cuprous Ions in a Copper Sulfate Plating Solution

Published on: March 20, 2019

15.0K
Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides
11:04

Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides

Published on: September 7, 2019

9.1K
Quantifying the Binding Interactions Between CuII and Peptide Residues in the Presence and Absence of Chromophores
11:38

Quantifying the Binding Interactions Between CuII and Peptide Residues in the Presence and Absence of Chromophores

Published on: April 5, 2022

2.4K

Area of Science:

  • Analytical Chemistry
  • Environmental Science

Background:

  • Developing synthesis-free colorimetric assays is challenging.
  • 3,3',5,5'-tetramethylbenzidine (TMB) oxidation is a common basis for colorimetric detection.

Purpose of the Study:

  • To establish a synergistic Cu2+/Cr6+-promoting strategy for TMB-based colorimetric detection of Cr6+.
  • To elucidate the mechanism of TMB oxidation acceleration by Cu2+ and Cr6+.

Main Methods:

  • In situ formation of TMB·+···Cu(I/II)···TMB charge transfer complex (TMC) intermediates.
  • Utilizing the synergetic effect of Cu2+ and Cr6+ for TMB oxidation.
  • Colorimetric detection of Cr6+.

Main Results:

  • The strategy achieved sensitive and specific colorimetric detection of Cr6+ with a limit of detection of 0.006 μM.
  • TMC intermediates were identified to reduce activation energy for TMB oxidation and play a role in H2O2-participated oxidation.
  • The method demonstrated practical application in real water samples.

Conclusions:

  • The synergistic Cu2+/Cr6+ strategy provides an effective, synthesis-free method for Cr6+ detection.
  • This work enhances understanding of colorimetric sensing mechanisms and offers opportunities for developing new detection platforms.