Abiotic Foldamer Quaternary Structures

Abiotic aromatic foldamer sequences have been previously shown to fold in helix-turn-helix motifs in organic solvents. Using simple computational tools, a new helix-turn-helix motif was designed that bears additional hydrogen bond donor OH groups to promote its aggregation into a genuine, trimeric, abiotic quaternary structure. This sequence was synthesized and its self-assembly in solution was investigated by Nuclear Magnetic Resonance (NMR), Circular Dichroism (CD) and Molecular Dynamics (MD) simulations. The existence of two stable discrete aggregates was evidenced, one assigned to the initially designed trimer, the other to a dimer including multiple water molecules. The two species may be quantitatively interconverted upon changing the water content of the solution or the temperature. These results represent important steps in the design of protein-like abiotic architectures.