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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Updated: Jun 14, 2025

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes
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Nitroreductase-Based "Turn-On" Fluorescent Probe for Bacterial Identification with Visible Features.

Buyue Zhang1, Huan Chen2, Lei Shi1

  • 1Hebei Key Laboratory of Medical-Industrial Integration Precision Medicine, College of Chemical Engineering, North China University of Science and Technology, Tangshan 063210, China.

ACS Sensors
|September 4, 2024
PubMed
Summary
This summary is machine-generated.

New fluorescent probes offer a visual method to identify key antibiotic-resistant bacteria like Escherichia coli. This approach aids in accurate diagnosis and effective treatment strategies against resistant pathogens.

Keywords:
clinical bacteriafluorescent probehemicyaninenitroreductasevisualization

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Area of Science:

  • Microbiology and Infectious Diseases
  • Analytical Chemistry
  • Biotechnology

Background:

  • Antibiotic resistance is a major global health threat, with six bacterial species causing significant mortality in 2019.
  • Accurate bacterial identification is crucial for effective antibiotic treatment, yet current methods like mass spectrometry can be complex.
  • There is an urgent need for rapid and accessible diagnostic tools for identifying pathogenic bacteria.

Purpose of the Study:

  • To develop and evaluate novel nitroreductase-based "turn-on" fluorescent probes (ETH1-NO and ETH2-NO) for bacterial identification.
  • To assess the probes' ability to differentiate between six leading antibiotic-resistant bacterial species.
  • To explore the potential of these probes for visual bacterial detection and fluorescence imaging.

Main Methods:

  • Synthesis and characterization of two nitroreductase-based fluorescent probes, ETH1-NO and ETH2-NO.
  • Testing the probes' detection limits (LODs) for nitroreductase enzymes (NTRs).
  • Evaluating the probes' response to Gram-positive and Gram-negative bacteria, including specific pathogenic species, and assessing visual color changes.

Main Results:

  • The probes ETH1-NO and ETH2-NO demonstrated effective detection of nitroreductase enzymes with low LODs (0.562 and 0.153 μg/mL, respectively).
  • The probes responded to both Gram-positive and Gram-negative bacteria, achieving a low LOD of 1.2 × 10^6 CFU/mL for Escherichia coli.
  • ETH1-NO exhibited distinct color changes for different bacterial species, enabling potential visual identification and showing promise for bacterial fluorescence imaging.

Conclusions:

  • Nitroreductase-based fluorescent probes (ETH1-NO and ETH2-NO) provide an alternative, potentially visual, method for identifying key pathogenic bacteria.
  • These probes offer a sensitive detection capability applicable to a range of bacteria, including those resistant to antibiotics.
  • The developed probes represent novel reagents for bacterial identification and imaging, contributing to improved diagnostics and research.