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TET3 Protein Represses Proliferation of the MG-63 Human Osteosarcoma Cell Line by Regulating DNA Demethylation: an

Shijun Hang1,2, Bingjun Cui3, Aichun Wei4,5

  • 1Department of Orthopedics, Haian Hospital of Traditional Chinese Medicine, Haian, China.

Folia Biologica
|September 4, 2024
PubMed

Insights

Levels of 5-hydroxymethylcytosine (5hmC) and TET3 protein are decreased in osteosarcoma (OS). Lower TET3 levels correlate with reduced survival, and manipulating TET3 affects cancer cell proliferation, suggesting 5hmC and TET3 as potential OS biomarkers.

Area of Science:

  • Epigenetics
  • Oncology
  • Biochemistry

Background:

  • 5-hydroxymethylcytosine (5hmC) plays a role in various cancers.
  • The specific function of 5hmC and TET3 in osteosarcoma (OS) is not well understood.

Purpose of the Study:

  • To investigate the role and levels of 5hmC and TET3 in osteosarcoma.
  • To determine the potential of 5hmC and TET3 as biomarkers for OS.

Main Methods:

  • Quantification of 5hmC and TET3 protein levels in OS tissues.
  • Manipulation of TET3 expression (silencing and overexpression) in MG-63 osteosarcoma cells.
  • Assessment of cell proliferation and invasion rates following TET3 level changes.

Main Results:

  • Osteosarcoma tissues exhibited decreased levels of both 5hmC and TET3 protein.
  • Reduced TET3 expression was associated with a lower disease-free survival (DFS) rate in patients.
  • Silencing TET3 in MG-63 cells increased proliferation and decreased 5hmC levels.
  • Overexpressing TET3 inhibited proliferation and invasion while increasing 5hmC levels.

Conclusions:

  • Both 5hmC and TET3 protein levels are significantly decreased in osteosarcoma.
  • TET3 plays a crucial role in regulating osteosarcoma cell proliferation and invasion.
  • Decreased 5hmC and TET3 may serve as potential diagnostic and prognostic biomarkers for osteosarcoma.
Keywords:
5hmCOsteosarcomaTET3

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