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Mitochondria

Mitochondria

Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...

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Related Experiment Video

Updated: Jul 21, 2026

Using Microfluidic Devices to Measure Lifespan and Cellular Phenotypes in Single Budding Yeast Cells

Using Microfluidic Devices to Measure Lifespan and Cellular Phenotypes in Single Budding Yeast Cells

Published on: March 30, 2017

A Single-Cell Metabolic Profiling Characterizes Human Aging via SlipChip-SERS.

Fugang Liu¹, Jiaqing Liu¹, Yang Luo¹

¹School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)

|September 4, 2024

Summary

A new SlipChip-SERS system analyzes single-cell metabolism, revealing spermine as a key inducer of cellular senescence. This discovery offers potential new strategies for combating aging and related diseases.

Keywords:

SlipChip aging metabolic profiling single‐cell surface‐enhanced Raman spectroscopy

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Last Updated: Jul 21, 2026

Using Microfluidic Devices to Measure Lifespan and Cellular Phenotypes in Single Budding Yeast Cells

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Published on: March 30, 2017

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Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

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Published on: April 6, 2022

Area of Science:

Biochemistry
Cell Biology
Aging Research

Background:

Metabolic dysregulation significantly drives cellular senescence and systemic aging.
The complex metabolic shifts and heterogeneity of senescent cells remain poorly understood.
Developing advanced tools for single-cell metabolic analysis is crucial for aging research.

Purpose of the Study:

To develop a novel microfluidic device integrated with SERS for single-cell metabolism analysis.
To investigate metabolic differences in cells and identify novel inducers of cellular senescence.
To explore the potential of spermine as an inducer of senescence and its implications for aging.

Main Methods:

Development of a microfluidic SlipChip device combined with surface-enhanced Raman spectroscopy (SERS) for single-cell analysis.
Compartmentalization of single cells and parallel delivery of reagents for metabolite release and SERS detection.
Application of the SlipChip-SERS system to cancer cell lines and human primary fibroblasts of varying ages.

Main Results:

The SlipChip-SERS system demonstrated sensitive and multiplexed metabolic profiling of individual cells.
Twelve differential metabolites were identified in aged fibroblasts, with spermine identified as a potent senescence inducer.
Spermine exposure induced classic senescence phenotypes, including increased senescence-associated β-galactosidase activity and altered gene expression, and its effects were confirmed in HUVECs and WRL-68 cells.

Conclusions:

A novel SlipChip-SERS system enables precise single-cell metabolic analysis.
Spermine is identified as a potential inducer of cellular senescence across multiple cell types.
This research may pave the way for new therapeutic strategies targeting aging and age-related diseases.