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Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

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Published on: July 25, 2022

Multiplexed digital colloid-enhanced Raman spectroscopy for metabolite detection via selective molecular affinity.

Shuang Ni¹, Zhewen Luo¹, Xinyuan Bi¹

¹State Key Laboratory of Systems Medicine for Cancer, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, P.R. China. yejian78@sjtu.edu.cn.

Chemical Communications (Cambridge, England)

|June 16, 2026

Summary

A novel dual-nanoparticle platform captures a wide range of metabolites. This technology, combined with digital colloid-enhanced Raman spectroscopy, allows for sensitive detection of multiple low-concentration compounds.

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Area of Science:

Analytical Chemistry
Biochemistry
Materials Science

Background:

Accurate metabolite quantification is crucial for understanding biological processes and disease states.
Current methods for multiplex metabolite analysis often face limitations in sensitivity and breadth of coverage.
Developing advanced analytical tools is essential for comprehensive metabolic profiling.

Purpose of the Study:

To develop a novel dual-nanoparticle platform for broad metabolite capture.
To integrate this platform with digital colloid-enhanced Raman spectroscopy (d-CEERS).
To achieve sensitive and multiplexed quantification of metabolites in complex, low-concentration mixtures.

Main Methods:

Construction of a dual-nanoparticle system with tailored, complementary affinities for diverse metabolites.
Integration of the nanoparticle platform with digital colloid-enhanced Raman spectroscopy (d-CEERS) for signal enhancement.
Application of the integrated system to analyze low-concentration metabolite mixtures.

Main Results:

The dual-nanoparticle platform demonstrated broad affinity for a wide spectrum of metabolites.
The integrated d-CEERS system achieved high sensitivity in metabolite detection.
Successful multiplex quantification of multiple metabolites in complex mixtures was demonstrated.

Conclusions:

The developed dual-nanoparticle platform coupled with d-CEERS offers a sensitive and versatile approach for metabolite analysis.
This integrated system enables efficient capture and detection of diverse metabolites, even at low concentrations.
The technology holds promise for advancing metabolic research and diagnostics.