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Quantification Of Osilodrostat In Horse Urine Using Lc/esi-hrms To Establish An Elimination Profile For Doping Control.

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Quantification Of Osilodrostat In Horse Urine Using Lc/esi-hrms To Establish An Elimination Profile For Doping Control.

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Quantification of osilodrostat in horse urine using LC/ESI-HRMS to establish an elimination profile for doping

Hideaki Ishii^1,2, Ryo Shigematsu¹, Shunsuke Takemoto¹

¹Drug Analysis Department, Laboratory of Racing Chemistry, 1731-2 Tsuruta-machi, Utsunomiya, Tochigi, 320-0851, Japan.

|September 5, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Osilodrostat, an anabolic agent banned in horses, can be detected in urine for up to two weeks. Monitoring its metabolite M1c and osilodrostat is recommended for effective equine doping control.

Keywords:

LC/ESI–HRMS anabolic agent doping control elimination profile equine osilodrostat quantitative analysis urine

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Area of Science:

Equine pharmacology
Doping control science
Analytical chemistry

Background:

Osilodrostat is an anabolic agent banned in horse racing by major international federations.
Accurate detection methods are crucial for enforcing anti-doping regulations in equestrian sports.

Purpose of the Study:

To establish elimination profiles of osilodrostat in horse urine.
To investigate the detectability of osilodrostat and its metabolite M1c for doping control.

Main Methods:

Analysis of post-administration horse urine samples using a validated method.
Enzymatic hydrolysis followed by LC/ESI-HRMS analysis.
Quantification with a lower limit of quantification (LLOQ) of 0.05 ng/ml.

Main Results:

Hydrolyzed osilodrostat was quantifiable in urine from 48 to 72 hours post-administration.
Both hydrolyzed osilodrostat and its metabolite M1c were detected for up to two weeks.
Confirmatory analysis detected hydrolyzed osilodrostat for up to 72 hours.

Conclusions:

Monitoring both hydrolyzed M1c and osilodrostat is recommended for equine doping control.
M1c offers greater detectability, while osilodrostat reference material is vital for confirmation.