Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

20 Years Later: The ALH 84001 Debate in Context with Viking's Results. Lessons Learned and Portals Opened.

Astrobiology·2026
Same author

Spatially distributed complex organic matter detected in an ancient river valley in Jezero crater, Mars.

Science advances·2026
Same author

The role of group III/IV afferent feedback in the ventilatory, pressor and metabolic responses to exercise in normoxia and hypoxia.

The Journal of physiology·2026
Same author

Mars Organic Geochemistry-Are We Alone, and Where Did We Come From?

Astrobiology·2026
Same author

Effects of Increasing the Concentration of Dialysate Magnesium on Cardiovascular Health: A Narrative Review.

Canadian journal of kidney health and disease·2026
Same author

Amplifying missing voices in healthcare research: an AI framework for co-production of PPIE.

Frontiers in digital health·2026

Related Experiment Video

Updated: Jun 22, 2026

Ratiometric Imaging of Extracellular pH in Dental Biofilms
13:05

Ratiometric Imaging of Extracellular pH in Dental Biofilms

Published on: March 9, 2016

10.3K

In Situ Quantification of Carbonate Species Concentrations, pH, and pCO2 in Calcite Fluid Inclusions Using Confocal

Michael Naylor Hudgins1,2,3, Todd K Knobbe1, Julia Hubbard1

  • 1Department of Earth and Environmental Sciences, Rensselaer Polytechnic Institute, Jonsson Rowland Science Center, Troy, New York, USA.

Applied Spectroscopy
|September 6, 2024
PubMed
Summary

This study introduces a new method using confocal Raman spectroscopy to precisely measure carbonate and bicarbonate ion concentrations and pH within fluid inclusions in calcite. This technique allows for detailed reconstruction of ancient geochemical conditions and paleoenvironments.

Keywords:
CO2Raman spectroscopycarbonate systemfluid inclusionspHquantification

More Related Videos

Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography
10:18

Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

Published on: February 21, 2017

8.5K
Fluid-cell Raman Spectroscopy for operando Studies of Reaction and Transport Phenomena during Silicate Glass Corrosion
06:48

Fluid-cell Raman Spectroscopy for operando Studies of Reaction and Transport Phenomena during Silicate Glass Corrosion

Published on: May 9, 2025

228

Related Experiment Videos

Last Updated: Jun 22, 2026

Ratiometric Imaging of Extracellular pH in Dental Biofilms
13:05

Ratiometric Imaging of Extracellular pH in Dental Biofilms

Published on: March 9, 2016

10.3K
Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography
10:18

Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

Published on: February 21, 2017

8.5K
Fluid-cell Raman Spectroscopy for operando Studies of Reaction and Transport Phenomena during Silicate Glass Corrosion
06:48

Fluid-cell Raman Spectroscopy for operando Studies of Reaction and Transport Phenomena during Silicate Glass Corrosion

Published on: May 9, 2025

228

Area of Science:

  • Geochemistry
  • Mineralogy
  • Spectroscopy

Background:

  • Carbonate minerals are widespread and preserve fluid geochemistry in inclusions.
  • Fluid inclusions offer insights into past pCO2 and paleoenvironments.
  • Confocal laser Raman spectroscopy is a non-destructive method for analyzing fluid inclusions.

Purpose of the Study:

  • To develop and validate a quantitative method for analyzing fluid inclusions in calcite.
  • To measure carbonate (CO3^2-) and bicarbonate (HCO3^-) ion concentrations and pH using confocal Raman spectroscopy.
  • To apply this method for paleoenvironmental reconstruction.

Main Methods:

  • Calibrated confocal laser Raman spectroscopy using stock solutions.
  • Quantified carbonate and bicarbonate ion concentrations and pH in calcite fluid inclusions.
  • Analyzed Icelandic spar calcite samples.

Main Results:

  • Accurate quantification of carbonate and bicarbonate concentrations down to 20 mM and pH from 7-11.
  • Demonstrated that the calcite host mineral does not interfere with measurements.
  • Measured specific concentrations ([CO3^2-]=0.11, [HCO3^-]=0.17, pH=10.1) and CO2 (2217 ppm) in Icelandic spar.
  • Identified CO2, CH4, and H2S, indicating a reducing environment.

Conclusions:

  • Confocal Raman spectroscopy provides a reliable method for quantifying key geochemical parameters in calcite fluid inclusions.
  • This technique enhances the ability to reconstruct past pCO2 and pH.
  • The findings suggest the Icelandic spar precipitated in a reducing environment.