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Updated: Jul 17, 2026

Electrophysiological Measurements from a Moth Olfactory System
Published on: March 29, 2011
Protocol for aerial trapping and analyses of candidate pheromone compounds released by moths via gas
Sagnik Ghosh1, Antonio Palazzo1, Jérémy Gévar1
1Institute of Ecology and Environmental Sciences of Paris (iEES-Paris), INRAE, Sorbonne University, CNRS, IRD, UPEC, Université Paris Cité, 78026 Versailles, France.
Abstract:
Detection of pheromones is pivotal to chemical ecology and agronomy; however, analytic detection of the volatile pheromone components from odorized air is highly challenging. Here, we introduce a protocol for the detection of airborne pheromones from female moths, which are key models for chemosensory studies. We describe a step-by-step guide from pheromone collection to quantitative estimation of pheromone components. We also detail procedures for gas chromatography-mass spectrometry (GC-MS) analysis. This protocol has potential applications beyond chemosensory research, particularly in environmental chemistry. For complete details on the use and execution of this protocol, please refer to Ghosh et al.1.
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