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Related Experiment Videos

A catabolin-like factor from porcine kidney.

A J Monserrat, J T Dingle

    Cell Biochemistry and Function
    |January 1, 1985
    PubMed
    Summary

    Pig kidney explants release a catabolin-like factor (CLF). This kidney CLF, found in both cortex and medulla, has a molecular weight of approximately 22,500 and acts on live cartilage.

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    Area of Science:

    • Nephrology
    • Endocrinology
    • Biochemistry

    Background:

    • Kidney explants from pigs, specifically the cortex and medulla, secrete a factor.
    • This secreted factor exhibits characteristics similar to catabolin.

    Purpose of the Study:

    • To characterize the catabolin-like factor (CLF) released by pig kidney tissues.
    • To investigate the bioactivity of kidney CLF on cartilage.
    • To explore the potential role of CLF as a local hormone in the kidney.

    Main Methods:

    • Ammonium sulphate precipitation was used to isolate CLF from kidney cortex and medulla explants.
    • Gel chromatography was employed to determine the molecular weight of the kidney CLF.
    • Bioassays were conducted using live and dead cartilage to assess CLF activity.
    • Glomeruli and dissociated glomerular cells were cultured to study CLF release.

    Main Results:

    • Catabolin-like factor (CLF) was successfully precipitated from both pig kidney cortex and medulla using ammonium sulphate (60-95% fraction).
    • Gel chromatography identified a major active fraction of kidney CLF with a molecular weight of approximately 22,500.
    • CLF released by whole glomeruli and glomerular cells demonstrated bioactivity on live cartilage but not on dead cartilage.

    Conclusions:

    • Pig kidney cortex and medulla produce and release a catabolin-like factor (CLF).
    • Kidney CLF has a distinct molecular weight and biological activity on live cartilage.
    • The findings suggest a potential local hormonal role for CLF within the kidney.

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