Related Experiment Video
Updated: Jun 14, 2025
09:58
Light-driven Enzymatic Decarboxylation
Published on: May 22, 2016
10.7K
H2-driven biocatalysis for flavin-dependent ene-reduction in a continuous closed-loop flow system utilizing H2 from
Guiyeoul Lim1, Donato Calabrese1, Allison Wolder2
1Institute of Applied Microbiology-iAMB RWTH Aachen University, Aachen, Germany.
Communications Chemistry
|September 7, 2024
Summary
We developed a scalable biocatalysis flow system for sustainable fine chemical production. This system efficiently reduces cyclic enones using immobilized enzymes and electro-driven hydrogen for cofactor regeneration.
Area of Science:
- Biocatalysis and Sustainable Chemistry
- Chemical Engineering and Process Development
Background:
- Scalable biocatalysis for fine chemical synthesis faces challenges.
- Efficient cofactor regeneration is crucial for flavin-dependent enzymes.
Purpose of the Study:
- To develop a scalable flow system for flavin-dependent biocatalysis.
- To demonstrate asymmetric alkene reduction using immobilized enzymes and electro-driven hydrogen.
Main Methods:
- Integrated immobilized Old Yellow Enzyme (OYE) and hydrogenase in a flow system.
- Utilized electro-generated H2 from a PEM electrolyzer for cofactor regeneration.
- Employed a gas membrane addition module for efficient H2 diffusion.
Main Results:
- Achieved >99% conversion of ketoisophorone to levodione.
- Demonstrated high stability and reusability of the flow system.
- Showcased versatility in reducing various cyclic enones.
Conclusions:
- The electro-driven continuous flow system is scalable and sustainable.
- This approach has significant potential for fine chemical synthesis.
- The system is adaptable for other flavin-based and gas-dependent biocatalytic reactions.
Related Concept Videos
03:00Electrolysis
26.2K
In a galvanic cell, the electrical work is done by a redox system on its surroundings as electrons produced by the spontaneous redox reactions are transferred through an external circuit. Alternatively, an external circuit does work on a redox system by imposing a voltage sufficient to drive an otherwise nonspontaneous reaction in a process known as electrolysis. For instance, recharging a battery involves the use of an external power source to drive the spontaneous (discharge) cell reaction in...
26.2K
02:13Reduction of Alkenes: Catalytic Hydrogenation
11.9K
Alkenes undergo reduction by the addition of molecular hydrogen to give alkanes. Because the process generally occurs in the presence of a transition-metal catalyst, the reaction is called catalytic hydrogenation.
Metals like palladium, platinum, and nickel are commonly used in their solid forms — fine powder on an inert surface. As these catalysts remain insoluble in the reaction mixture, they are referred to as heterogeneous catalysts.
The hydrogenation process takes place on the...
Metals like palladium, platinum, and nickel are commonly used in their solid forms — fine powder on an inert surface. As these catalysts remain insoluble in the reaction mixture, they are referred to as heterogeneous catalysts.
The hydrogenation process takes place on the...
11.9K
02:17Reduction of Alkenes: Asymmetric Catalytic Hydrogenation
3.3K
Catalytic hydrogenation of alkenes is a transition-metal catalyzed reduction of the double bond using molecular hydrogen to give alkanes. The mode of hydrogen addition follows syn stereochemistry.
The metal catalyst used can be either heterogeneous or homogeneous. When hydrogenation of an alkene generates a chiral center, a pair of enantiomeric products is expected to form. However, an enantiomeric excess of one of the products can be facilitated using an enantioselective reaction or an...
The metal catalyst used can be either heterogeneous or homogeneous. When hydrogenation of an alkene generates a chiral center, a pair of enantiomeric products is expected to form. However, an enantiomeric excess of one of the products can be facilitated using an enantioselective reaction or an...
3.3K
01:29Role of Reduced Coenzymes NADH and FADH₂
11.3K
The energy released from the breakdown of the chemical bonds within nutrients can be stored either through the reduction of electron carriers or in the bonds of adenosine triphosphate (ATP). In living systems, a small class of compounds functions as mobile electron carriers, molecules that bind to and shuttle high-energy electrons between compounds in pathways. The principal electron carriers that will be considered originate from the B vitamin group and are derivatives of nucleotides; they are...
11.3K
01:32Chemiosmosis
97.3K
Oxidative phosphorylation is a highly efficient process that generates large amounts of adenosine triphosphate (ATP), the basic unit of energy that drives many cellular processes. Oxidative phosphorylation involves two processes— the electron transport chain and chemiosmosis.
Electron Transport Chain
The electron transport chain involves a series of protein complexes on the inner mitochondrial membrane that undergo a series of redox reactions. At the end of this chain, the electrons...
Electron Transport Chain
The electron transport chain involves a series of protein complexes on the inner mitochondrial membrane that undergo a series of redox reactions. At the end of this chain, the electrons...
97.3K
03:12Batteries and Fuel Cells
27.2K
A battery is a galvanic cell that is used as a source of electrical power for specific applications. Modern batteries exist in a multitude of forms to accommodate various applications, from tiny button batteries such as those that power wristwatches to the very large batteries used to supply backup energy to municipal power grids. Some batteries are designed for single-use applications and cannot be recharged (primary cells), while others are based on conveniently reversible cell reactions that...
27.2K